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Anti-RELA / NFKB p65 Antibody (C-Terminus, clone 27F9.G4) LS-C153771

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular RELA / NFKB p65 Antibodies

Anti-RELA / NFKB p65 Antibody (phospho-Ser529) IHC-plus™ LS-B652
Rabbit Polyclonal to Human RELA / NFKB p65
Human, Monkey, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RELA / NFKB p65 Antibody (aa526-539, clone 112A1021) IHC-plus™ LS-B6438
Mouse Monoclonal [clone 112A1021] (IgG1,k) to Human RELA / NFKB p65
Human, Monkey, Mouse, Bovine, Dog, Pig
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone 27F9.G4] (IgG) to Human RELA / NFKB p65
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human RELA / NFKB p65
Mouse
Human (tested or 100% immunogen sequence identity)
IgG Monoclonal [27F9.G4]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC (1:200 - 1:600)
  • Immunofluorescence (1:5000)
  • Western blot (1:1000 - 1:5000)
  • ELISA

Specificity and Use

RELA / NFKB p65 antibody was raised against nFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
C-Terminus
Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.
Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 (Rel A) and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

1
Her2 activates NF-kappaB and induces invasion through the canonical pathway involving IKKalpha. Merkhofer EC, Cogswell P, Baldwin AS. Oncogene. 2010 29:1238-48. [PubMed:19946332] [PMC:PMC2829103]

About RELA / NFKB p65

Q04206 NM_021975 NP_068810.3

RELA Antibody, NF-kappa-B p65delta3 Antibody, NFKB3 Antibody, p65 Antibody, Transcription factor p65 Antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Immunocytochemistry

Immunocytochemistry
Monoclonal anti NFKB p65 (Rel A) antibody was used to detect p65 by immunofluorescence at a dilution of 1:5000. HeLa cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and Triton X 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in wide field, confocal and STED CW imaging on the same system.

Western blot

Western blot
anti NFKB p65 (Rel A) monoclonal antibody LS-C153771 was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TTBS 30 min RT. Blot was probed with monoclonal anti p65 1:1000 in 1% BSA-TBS-T o/n 4°C and detected with HRP conjugated Rb-anti Mouse antibody 1:40000 in MB-070 30 min RT.

Requested From: United States
Date Requested: 12/3/2016

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