LSBio The Immunohistochemistry Antibody Company
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All ELISA Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-RELA / NFKB p65 Antibody (C-Terminus, clone 27F9.G4) LS-C153771


Wt. Vol. Conc. Price
100 µg - - Unavailable

Most Popular RELA / NFKB p65 Antibodies

Anti-RELA / NFKB p65 Antibody IHC-plus™ LS-B53
Rabbit Polyclonal to Human RELA / NFKB p65
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-RELA / NFKB p65 Antibody (phospho-Ser529) IHC-plus™ LS-B652
Rabbit Polyclonal to Human RELA / NFKB p65
Human, Monkey, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Immunohistochemistry Image
Anti-RELA / NFKB p65 Antibody (Ser536) IHC-plus™ LS-B1488
Rabbit Polyclonal (IgG) to Human RELA / NFKB p65
Human, Mouse, Rat
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-RELA / NFKB p65 Antibody LS-C88763
Rabbit Polyclonal (IgG) to Human RELA / NFKB p65
Human, Rat, Rabbit
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-RELA / NFKB p65 Antibody (Acetyl-Lys122) LS-C387648
Rabbit Polyclonal (IgG) to Human RELA / NFKB p65
Human, Mouse, Rat
Western blot, ELISA
Western blot Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone 27F9.G4] (IgG) to Human RELA / NFKB p65
IHC, Immunofluorescence, Western blot, ELISA


Human RELA / NFKB p65
Human (tested or 100% immunogen sequence identity)
IgG Monoclonal [27F9.G4]
Protein A purified


  • IHC (1:200 - 1:600)
  • Immunofluorescence (1:5000)
  • Western blot (1:1000 - 1:5000)

Specificity and Use

RELA / NFKB p65 antibody was raised against nFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.
Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 (Rel A) and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

Her2 activates NF-kappaB and induces invasion through the canonical pathway involving IKKalpha. Merkhofer EC, Cogswell P, Baldwin AS. Oncogene. 2010 29:1238-48. [PubMed:19946332] [PMC:PMC2829103]

About RELA / NFKB p65

Q04206 NM_021975 NP_068810.3

RELA Antibody, NF-kappa-B p65delta3 Antibody, NFKB3 Antibody, p65 Antibody, Transcription factor p65 Antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.


Monoclonal anti NFKB p65 (Rel A) antibody was used to detect p65 by immunofluorescence at a dilution of 1:5000. HeLa cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and Triton X 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in wide field, confocal and STED CW imaging on the same system.

Western blot

Western blot
anti NFKB p65 (Rel A) monoclonal antibody LS-C153771 was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TTBS 30 min RT. Blot was probed with monoclonal anti p65 1:1000 in 1% BSA-TBS-T o/n 4°C and detected with HRP conjugated Rb-anti Mouse antibody 1:40000 in MB-070 30 min RT.

Requested From: 
Date Requested: 4/25/2017

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2017 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number