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Catalog Number	Size	Price
LS-C153771-100	100 µg (0.98 mg/ml)	$574

RELA / NFKB p65 Antibody - Monoclonal anti NFKB p65 (Rel A) antibody was used to detect p65 by immunofluorescence at a dilution of 1:5000. HeLa cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and Triton X 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in wide field, confocal and STED CW imaging on the same system.

RELA / NFKB p65 Antibody - anti NFKB p65 (Rel A) monoclonal antibody Anti-NFKB p65 (Rel A) (MOUSE) Monoclonal Antibody - 200-301-065 was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TTBS 30 min RT. Blot was probed with monoclonal anti p65 1:1000 in 1% BSA-TBS-T o/n 4? and detected with HRP conjugated Rb-anti Mouse antibody 1:40000 in MB-070 30 min RT.

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Monoclonal Mouse anti‑Human RELA / NFKB p65 Antibody (clone 27F9.G4, C‑Terminus, IHC, IF, WB) LS‑C153771

Name: Anti-RELA / NFKB p65 Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C153771-100
Price: 574.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

RELA / NFKB p65 Mouse anti-Human Monoclonal (C-Terminus) (27F9.G4) Antibody

Application:

IHC, ICC, IF, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$574

LS-C153771-100

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

RELA / NFKB p65 Mouse anti-Human Monoclonal (C-Terminus) (27F9.G4) Antibody

Application:

IHC, ICC, IF, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

NFKB p65 antibody LS-C153771 is an unconjugated mouse monoclonal antibody to human NFKB p65 (RELA) (C-Terminus). Validated for ELISA, ICC, IF, IHC and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human RELA / NFKB p65

Synonyms

See All RELA / NFKB p65 Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG2a,k Monoclonal

Clone

27F9.G4

Conjugations

Unconjugated

Purification

Protein A affinity chromatography

Modifications

Unmodified

Immunogen

NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Epitope

C-Terminus

Applications

IHC (1:200 - 1:600)
ICC
Immunofluorescence (1:5000)
Western blot (1:1000 - 1:5000)
ELISA (1:50000 - 1:100000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 (Rel A) and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About RELA / NFKB p65

Uniprot: Q04206 NCBI: NM_021975 NP_068810.3

LSBio Ratings

RELA / NFKB p65 Antibody (C-Terminus, clone 27F9.G4) for IHC, ICC, IF/Immunofluorescence, WB/Western, ELISA LS-C153771 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Her2 activates NF-kappaB and induces invasion through the canonical pathway involving IKKalpha. Merkhofer EC, Cogswell P, Baldwin AS. Oncogene. 2010 29:1238-48.

PubMed: 19946332 PMC: PMC2829103

Submit a Publication for LS-C153771