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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RECQ5 / RECQL5 Antibody (aa431‑480) LS‑C40302
RECQL5 antibody LS-C40302 is an unconjugated rabbit polyclonal antibody to RECQL5 (RECQ5) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C40302-50
50 µg (1 mg/ml)
Unavailable

Popular RECQ5 / RECQL5 Products

Detection of Human RECQ5 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RECQ5 used at a dilution of 1:250.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence
Detection of Human RECQ5 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RECQ5 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
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Species: Human, Mouse, Dog
Applications: Western blot, Immunoprecipitation
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Species: Human
Applications: Western blot, Immunoprecipitation
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Species: Human
Applications: Western blot, ELISA

Product Description

RECQL5 antibody LS-C40302 is an unconjugated rabbit polyclonal antibody to RECQL5 (RECQ5) from human, mouse, rat and other species. Validated for WB.
About RECQ5 / RECQL5
Isoform beta is a DNA helicase that plays an important role in DNA replication, transcription and repair. Inhibits elongation of stalled transcripts at DNA damage sites by binding to the RNA polymerase II subunit POLR2A and blocking the TCEA1 binding site. Required for mitotic chromosome separation after cross-over events and cell cycle progress. Required for efficient DNA repair, including repair of inter-strand cross-links. Stimulates DNA decatenation mediated by TOP2A. O94762 NM_004259 NP_004250.4

RECQL5 Antibody, ATP-dependent DNA helicase Q5 Antibody, DNA helicase, RecQ-like type 5 Antibody, RecQ protein-like 5 Antibody, RECQ5 Antibody


Specifications

Target
Human RECQ5 / RECQL5
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Horse, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
RECQ5 / RECQL5 antibody was raised against synthetic peptide located between aa431-480 of human RECQL5 (Q6P4G0, NP_004250). Percent identity by BLAST analysis: Human, Xenopus (100%); Gorilla, Orangutan, Gibbon, Monkey, Marmoset (92%); Mouse, Rat, Elephant, Panda, Bovine, Bat, Rabbit, Horse (85%).
Blocking Peptide
LS-E13016 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C40302. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa431-480
Specificity
Human RECQL5
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RECQL5 antibody LS-C40302 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RECQL5 antibody LS-C40302 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RECQL5 antibody LS-C40302 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RECQL5 antibody LS-C40302 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RECQL5 antibody LS-C40302 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RECQL5 antibody LS-C40302 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RECQL5 antibody LS-C40302 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RECQL5 antibody LS-C40302 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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