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Anti-RBPJ Antibody (aa208-500) LS-C36026

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $635
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RBPJ Antibodies

Anti-RBPJ Antibody (aa1-29) LS-C158830
Rabbit Polyclonal to Human RBPJ
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RBPJ Antibody (aa331-380) IHC-plus™ LS-B14019
Rabbit Polyclonal to Mouse RBPJ
Mouse, Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human RBPJ
Human
ICC, Western blot, ELISA
Unconjugated

Details

Human RBPJ
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC
  • Western blot
  • ELISA

Specificity and Use

RBPJ antibody was raised against e. coli-derived rhCSL (aa208-500).
aa208-500
Selected for its ability to recognize human CSL in the applications listed.
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human CSL. The detection limit for rhCSL is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody can be used at a concentration of 10 ug/ml to detect human CSL on HASMC (human aortic smooth muscle) cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human CSL. The detection limit for rhCSL is approximately 5 ng/well.

Packaging

Lyophilized.
100 µl deionized water
For research use only.

About RBPJ

Q06330 NM_015874 NP_056958.3

RBPJ Antibody, AOS3 Antibody, CBF1 Antibody, Csl Antibody, KBF2 Antibody, H-2K binding factor-2 Antibody, RBP-JK Antibody, SUH Antibody, Suppressor of hairless homolog Antibody, RBP-J Antibody, RBP-J kappa Antibody, RBPJK Antibody, RBPSUH Antibody, CBF-1 Antibody, IGKJRB Antibody, IGKJRB1 Antibody

Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes.

Requested From: United States
Date Requested: 12/10/2016

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