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Anti-RBPJ Antibody (aa1-29) LS-C158830

Catalog Size Price
LS-C158830-400 400 µl Unavailable

Related Products

35 RBPJ Antibodies

Most Popular RBPJ Antibodies

Anti-RBPJ Antibody LS-C75345
Mouse Monoclonal (IgG1) to Human RBPJ
Human
Western blot
Unconjugated
Anti-RBPJ Antibody (aa1-29) LS-C158830
Rabbit Polyclonal to Human RBPJ
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
RBPJ Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Anti-RBPJ Antibody LS-C334209
Rabbit Polyclonal (IgG) to Human RBPJ
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry of paraffin-embedded Human gastric cancer tissue.
Anti-RBPJ Antibody (aa331-380) IHC-plus™ LS-B14019
Rabbit Polyclonal to Mouse RBPJ
Mouse, Human
IHC, IHC - Paraffin, Western blot
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Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-RBPJ Antibody LS-C679300
Rabbit Polyclonal (IgG) to Human RBPJ
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IHC, Immunofluorescence, Western blot, ELISA
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Immunohistochemistry of paraffin-embedded human pancreatic tissue at dilution of 1:100

100% Guaranteed Publications (1)
Rabbit Polyclonal to Human RBPJ
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human RBPJ
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

LS-E5001 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C158830. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa1-29
This RBPJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human RBPJ.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

1
RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation. Xie Q, Wu Q, Kim L, Miller TE, Liau BB, Mack SC, Yang K, Factor DC, Fang X, Huang Z, Zhou W, Alazem K, Wang X, Bernstein BE, Bao S, Rich JN. The Journal of clinical investigation. 2016 126:2757-72. (WB; Human) [Full Text Article] [PubMed:27322055] [PMC:PMC4922685]

About RBPJ

Q06330 NM_015874 NP_056958.3

RBPJ Antibody, AOS3 Antibody, CBF1 Antibody, Csl Antibody, KBF2 Antibody, H-2K binding factor-2 Antibody, RBP-JK Antibody, SUH Antibody, Suppressor of hairless homolog Antibody, RBP-J Antibody, RBP-J kappa Antibody, RBPJK Antibody, RBPSUH Antibody, CBF-1 Antibody, IGKJRB Antibody, IGKJRB1 Antibody

Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes.

Immunohistochemistry

RBPJ Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
RBPJ Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Fluorescent confocal image of A2058 cell stained with RBPJ Antibody. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.
Fluorescent confocal image of A2058 cell stained with RBPJ Antibody. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.

Western blot

RBPJ Antibody western blot of Ramos cell line lysates (35 ug/lane). The RBPJ antibody detected the RBPJ protein (arrow).
RBPJ Antibody western blot of Ramos cell line lysates (35 ug/lane). The RBPJ antibody detected the RBPJ protein (arrow).

Requested From: 
Date Requested: 4/25/2018

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