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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RBPJ Antibody (aa1‑29) LS‑C158830
RBPJ antibody LS-C158830 is an unconjugated rabbit polyclonal antibody to human RBPJ. Validated for IF, IHC and WB. Cited in 1 publication.
Catalog
Size
Price
LS-C158830-400
400 µl
Unavailable

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Product Description

RBPJ antibody LS-C158830 is an unconjugated rabbit polyclonal antibody to human RBPJ. Validated for IF, IHC and WB. Cited in 1 publication.
About RBPJ
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Q06330 NM_015874 NP_056958.3

RBPJ Antibody, AOS3 Antibody, CBF1 Antibody, Csl Antibody, KBF2 Antibody, H-2K binding factor-2 Antibody, RBP-JK Antibody, SUH Antibody, Suppressor of hairless homolog Antibody, RBP-J Antibody, RBP-J kappa Antibody, RBPJK Antibody, RBPSUH Antibody, CBF-1 Antibody, IGKJRB Antibody, IGKJRB1 Antibody


Specifications

Target
Human RBPJ
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E5001 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C158830. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1-29
Specificity
This RBPJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human RBPJ.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation. Xie Q, Wu Q, Kim L, Miller TE, Liau BB, Mack SC, Yang K, Factor DC, Fang X, Huang Z, Zhou W, Alazem K, Wang X, Bernstein BE, Bao S, Rich JN. The Journal of clinical investigation. 2016 126:2757-72. (WB; Human) [Full Text Article] [PubMed:27322055] [PMC:PMC4922685]

Customer Reviews (0)


Images


Immunohistochemistry

RBPJ Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
RBPJ Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Fluorescent confocal image of A2058 cell stained with RBPJ Antibody. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.
Fluorescent confocal image of A2058 cell stained with RBPJ Antibody. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.

Western blot

RBPJ Antibody western blot of Ramos cell line lysates (35 ug/lane). The RBPJ antibody detected the RBPJ protein (arrow).
RBPJ Antibody western blot of Ramos cell line lysates (35 ug/lane). The RBPJ antibody detected the RBPJ protein (arrow).

Immunohistochemistry

RBPJ Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
RBPJ Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Fluorescent confocal image of A2058 cell stained with RBPJ Antibody. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.
Fluorescent confocal image of A2058 cell stained with RBPJ Antibody. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.

Western blot

RBPJ Antibody western blot of Ramos cell line lysates (35 ug/lane). The RBPJ antibody detected the RBPJ protein (arrow).
RBPJ Antibody western blot of Ramos cell line lysates (35 ug/lane). The RBPJ antibody detected the RBPJ protein (arrow).

Immunohistochemistry

RBPJ Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
RBPJ Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Fluorescent confocal image of A2058 cell stained with RBPJ Antibody. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.
Fluorescent confocal image of A2058 cell stained with RBPJ Antibody. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.

Western blot

RBPJ Antibody western blot of Ramos cell line lysates (35 ug/lane). The RBPJ antibody detected the RBPJ protein (arrow).
RBPJ Antibody western blot of Ramos cell line lysates (35 ug/lane). The RBPJ antibody detected the RBPJ protein (arrow).

Immunohistochemistry

RBPJ Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
RBPJ Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Fluorescent confocal image of A2058 cell stained with RBPJ Antibody. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.
Fluorescent confocal image of A2058 cell stained with RBPJ Antibody. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.

Western blot

RBPJ Antibody western blot of Ramos cell line lysates (35 ug/lane). The RBPJ antibody detected the RBPJ protein (arrow).
RBPJ Antibody western blot of Ramos cell line lysates (35 ug/lane). The RBPJ antibody detected the RBPJ protein (arrow).


Requested From: 
Date Requested: 6/18/2018

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