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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RBP4 Antibody (N‑Terminus) LS‑C107513
RBP4 antibody LS-C107513 is an unconjugated rabbit polyclonal antibody to RBP4 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C107513-50
50 µg
Unavailable

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Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
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Product Description

RBP4 antibody LS-C107513 is an unconjugated rabbit polyclonal antibody to RBP4 from human, mouse, rat and other species. Validated for WB.
About RBP4
RBP4 belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. P02753 NM_006744 NP_006735.2

RBP4 Antibody, PRBP Antibody, Plasma retinol-binding protein Antibody, RBP Antibody, Retinol-binding protein 4 Antibody


Specifications

Target
Human RBP4
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Bat, Bovine, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
RBP4 antibody was raised against synthetic peptide from N-Terminus of human RBP4 (P02753, NP_006735). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Horse, Pig (100%); Elephant, Bovine, Bat, Rabbit, Opossum (92%); Galago (91%); Guinea pig (85%); Turkey, Zebra finch, Chicken (84%).
Blocking Peptide
LS-E15306 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C107513. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human RBP4
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RBP4 antibody LS-C107513 Western blot of Fetal Stomach lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBP4 antibody LS-C107513 Western blot of Fetal Stomach lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RBP4 antibody LS-C107513 Western blot of Fetal Stomach lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBP4 antibody LS-C107513 Western blot of Fetal Stomach lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RBP4 antibody LS-C107513 Western blot of Fetal Stomach lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBP4 antibody LS-C107513 Western blot of Fetal Stomach lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RBP4 antibody LS-C107513 Western blot of Fetal Stomach lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBP4 antibody LS-C107513 Western blot of Fetal Stomach lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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