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Catalog Number	Size	Price
LS-C139674-100	100 µg (0.5 mg/ml)	$503

RBM20 Antibody - RBM20 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing njuclear and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

RBM20 Antibody - RBM20 (aa717-727) Antibody (0.3µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer) Detected by chemiluminescencence.

RBM20 Antibody - RBM20 antibody (1µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.

RBM20 Antibody - RBM20 antibody (1 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

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Polyclonal Goat anti‑Human RBM20 Antibody (aa717‑727, WB) LS‑C139674

Name: Anti-RBM20 Antibody | Goat anti-Human Polyclonal Peptide-ELISA,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C139674-100
Price: 503.0000 USD
Availability: InStock

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Antibody:

RBM20 Goat anti-Human Polyclonal (aa717-727) Antibody

Application:

WB, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse

Format:

Unconjugated, Unmodified

$503

LS-C139674-100

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

RBM20 Goat anti-Human Polyclonal (aa717-727) Antibody

Application:

WB, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

RBM20 antibody LS-C139674 is an unconjugated goat polyclonal antibody to RBM20 (aa717-727) from human. It is reactive with human, mouse and monkey. Validated for Peptide-ELISA and WB.

Target

Human RBM20

Synonyms

RBM20 | RNA-binding motif protein 20 | RNA binding motif protein 20

See All RBM20 Antibodies

Host

Goat

Reactivity

Human, Monkey, Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-QLDKAELDERP, from the internal region of the protein sequence according to NP_001127835.2.

Epitope

aa717-727

Specificity

Human RBM20.

Applications

Western blot (0.3 - 1 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:64000)

Usage

Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 150kD band observed in Mouse Heart lysates (calculated MW of 130kD according to Mouse NP_001164318.1). Recommended concentration: 1-3 ug/ml. An additional band of 25kD was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About RBM20

Uniprot: Q5T481 NCBI: Q5T481