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Catalog Number Size Price
LS-C154242-100 100 µg (1.39 mg/ml) $567 
RBL2 / RB2 p130 Antibody - Anti-Spa310 Antibody - Western Blot. Western blot of affinity purified anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kD, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4C. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4C. ECL was used for detection. Personal communication, Ang Sun, Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, PA.

Polyclonal Rabbit anti‑Human RBL2 / RB2 p130 Antibody (WB) LS‑C154242

Polyclonal Rabbit anti‑Human RBL2 / RB2 p130 Antibody (WB) LS‑C154242

Antibody:
RBL2 / RB2 p130 Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C154242-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RBL2 / RB2 p130 Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified

Specifications

Description
RB2 p130 antibody LS-C154242 is an unconjugated rabbit polyclonal antibody to RB2 p130 (RBL2) from human. It is reactive with human and chimpanzee. Validated for ELISA, IP and WB.
Target
Human RBL2 / RB2 p130
Synonyms
RBL2 | p130 | Retinoblastoma-like 2 (p130) | RBR-2 | Rb2 | Retinoblastoma-like protein 2
Host
Rabbit
Reactivity
Human, Chimpanzee (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein.  A residue of cysteine was added to facilitate coupling.
Specificity
This antiserum is directed against Spa310 and reacts with the Spa310 domain of pRb2/p130 from human tissues. Based on the sequence we expect this antibody to react as well Spa310 from chimpanzee and orangutan, and with lesser affinity, Spa310 from horse, dog, bovine, rat and opossum.
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:75000 - 1:100000)
Usage
This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kD in size corresponding to pRb2/p130, and a band approximately 4.2kD in size corresponding to Spa310 peptide (latter not shown), by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RBL2 / RB2 p130
Q08999 NM_005611 NP_005602.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024