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RBFOX2 / RBM9 Antibody (aa287‑336) LS‑C110564
RBM9 antibody LS-C110564 is an unconjugated rabbit polyclonal antibody to RBM9 (RBFOX2) from human and monkey. Validated for WB.
Catalog
Size
Price
LS-C110564-50
50 µg
Unavailable

Popular RBFOX2 / RBM9 Products

RBFOX2 / RBM9 antibody LS-C116098 Western blot of Mouse Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
Applications: Western blot
Anti-RBFOX2 / RBM9 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6218 concentration 3 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Detection of Mouse RBM9 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-RBM9 used at a dilution of 1:250.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence
Detection of Human and Mouse Immunohistochemistry. Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-No. Lot2) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry image at a dilution of 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications:

Product Description

RBM9 antibody LS-C110564 is an unconjugated rabbit polyclonal antibody to RBM9 (RBFOX2) from human and monkey. Validated for WB.
About RBFOX2 / RBM9
RBFOX2 / RBM9 is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. O43251 NM_014309 NP_055124.1

RBFOX2 Antibody, DJ106I20.3 Antibody, Fox-2 Antibody, Fxh Antibody, Fox-1 homolog B Antibody, HRNBP2 Antibody, Fox-1 homologue Antibody, HNRBP2 Antibody, RNA-binding motif protein 9 Antibody, RNA-binding protein 9 Antibody, RBM9 Antibody, FOX2 Antibody, RNA binding motif protein 9 Antibody, RTA Antibody


Specifications

Target
Human RBFOX2 / RBM9
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
RBFOX2 / RBM9 antibody was raised against synthetic peptide located between aa287-336 of human RBFOX2 (O43251-9, NP_055124). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey (100%); Mouse, Rat (92%); Horse (84%); Drosophila (83%).
Blocking Peptide
LS-E14941 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110564. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa287-336
Specificity
Human RBFOX2 / RBM9
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RBFOX2 / RBM9 antibody LS-C110564 Western blot of NCI-H226 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBFOX2 / RBM9 antibody LS-C110564 Western blot of NCI-H226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RBFOX2 / RBM9 antibody LS-C110564 Western blot of NCI-H226 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBFOX2 / RBM9 antibody LS-C110564 Western blot of NCI-H226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RBFOX2 / RBM9 antibody LS-C110564 Western blot of NCI-H226 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBFOX2 / RBM9 antibody LS-C110564 Western blot of NCI-H226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RBFOX2 / RBM9 antibody LS-C110564 Western blot of NCI-H226 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBFOX2 / RBM9 antibody LS-C110564 Western blot of NCI-H226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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