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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RASGRP2 Antibody (aa7‑34) LS‑C162459
RASGRP2 antibody LS-C162459 is an unconjugated rabbit polyclonal antibody to human RASGRP2. Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C162459-400
400 µl
Unavailable

Popular RASGRP2 Products

RASGRP2 antibody LS-C112261 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Rat, Dog, Guinea pig
Applications: Western blot
Antibody
Species: Human
Applications: ELISA
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Anti-RASGRP2 Antibody (N-term) at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

RASGRP2 antibody LS-C162459 is an unconjugated rabbit polyclonal antibody to human RASGRP2. Validated for IF, IHC and WB.
About RASGRP2
Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. NM_005825

RASGRP2 Antibody, CALDAG-GEFI Antibody, Cdc25-like protein Antibody, CDC25L Antibody, HCDC25L Antibody, MCG7 Antibody, Ras activator rasgrp Antibody, RAS guanyl-releasing protein 2 Antibody, F25B3.3 kinase-like protein Antibody, Guanine exchange factor MCG7 Antibody


Specifications

Target
Human RASGRP2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E1475 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C162459. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa7-34
Specificity
This RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human RASGRP2.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

RASGRP2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
RASGRP2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of RASGRP2 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of RASGRP2 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

RASGRP2 Antibody western blot of K562 cell line lysates (35 ug/lane). The RASGRP2 antibody detected the RASGRP2 protein (arrow).
RASGRP2 Antibody western blot of K562 cell line lysates (35 ug/lane). The RASGRP2 antibody detected the RASGRP2 protein (arrow).

Immunohistochemistry

RASGRP2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
RASGRP2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of RASGRP2 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of RASGRP2 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

RASGRP2 Antibody western blot of K562 cell line lysates (35 ug/lane). The RASGRP2 antibody detected the RASGRP2 protein (arrow).
RASGRP2 Antibody western blot of K562 cell line lysates (35 ug/lane). The RASGRP2 antibody detected the RASGRP2 protein (arrow).

Immunohistochemistry

RASGRP2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
RASGRP2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of RASGRP2 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of RASGRP2 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

RASGRP2 Antibody western blot of K562 cell line lysates (35 ug/lane). The RASGRP2 antibody detected the RASGRP2 protein (arrow).
RASGRP2 Antibody western blot of K562 cell line lysates (35 ug/lane). The RASGRP2 antibody detected the RASGRP2 protein (arrow).

Immunohistochemistry

RASGRP2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
RASGRP2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of RASGRP2 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of RASGRP2 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

RASGRP2 Antibody western blot of K562 cell line lysates (35 ug/lane). The RASGRP2 antibody detected the RASGRP2 protein (arrow).
RASGRP2 Antibody western blot of K562 cell line lysates (35 ug/lane). The RASGRP2 antibody detected the RASGRP2 protein (arrow).


Requested From: 
Date Requested: 6/23/2018

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