Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
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immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
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IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Mouse Monoclonal [clone 2D2] (IgG1,k) to Human RARA / RAR Alpha
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Details
Human RARA / RAR Alpha
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [2D2]
Unconjugated
Purified from ascites by Protein A
Unmodified
Applications
IHC
IHC - Paraffin (5 µg/ml)
Western blot
ELISA
Specificity and Use
RARA (NP_000955, 315 a.a. ~ 425 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human RARA
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
Packaging
PBS, pH 7.4.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5.
Anti-RARA antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5525 concentration 5 ug/ml.
Western blot
RARA monoclonal antibody clone 2D2 Western blot of RARA expression in HeLa NE.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RARA is approximately 1 ng/ml as a capture antibody.