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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAPGEF1 Antibody (phospho‑Tyr504) LS‑C199518
RAPGEF1 antibody LS-C199518 is an unconjugated rabbit polyclonal antibody to human RAPGEF1. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C199518-50
50 µl
Unavailable
LS-C199518-100
100 µl
Unavailable

Popular RAPGEF1 Products

Anti-RAPGEF1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RAPGEF1.
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IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Species: Human, Monkey, Mouse, Rat, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
IHC of paraffin-embedded Human pancreas tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
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Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescent staining of HeLa cells using anti-RAPGEF1 mouse monoclonal antibody.
Species: Human, Monkey
Applications: Immunofluorescence, Western blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot

Product Description

RAPGEF1 antibody LS-C199518 is an unconjugated rabbit polyclonal antibody to human RAPGEF1. Validated for Peptide-ELISA and WB.
About RAPGEF1
GRF2 is a human guanine nucleotide releasing protein for Ras protein. It belongs to the adaptor-type Src homology (SH)2-containing molecules. Src homology 2 domains are globular protein modules present in a large variety of functionally distinct proteins. They mediate binding events that control the activity and localization of many proteins involved in the transmission of signals from the cell surface to the nucleus. Q13905 NM_005312 NP_005303.2

RAPGEF1 Antibody, C3G Antibody, CRK SH3-binding GNRP Antibody, GRF2 Antibody, Protein C3G Antibody


Specifications

Target
Human RAPGEF1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Immunogen
RAPGEF1 antibody was raised against synthetic peptide derived from human RapGEF1 around the phosphorylation site of Tyr504.
Epitope
pTyr504
Specificity
RapGEF1 (Phospho-Tyr504) Antibody detects endogenous levels of RapGEF1 only when phosphorylated at tyrosine504.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of extracts from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/23/2018

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