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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAP2 Antibody (aa31‑80) LS‑C151601
RAP2 antibody LS-C151601 is an unconjugated rabbit polyclonal antibody to RAP2 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C151601-50
50 µg (1 mg/ml)
Unavailable

Popular RAP2 Products

Antibody
Species: Human, Monkey, Mouse, Dog, Horse, Xenopus
Applications: IHC, Western blot
RAP2 antibody Western Blot of MCF7.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Bat
Applications: Western blot
Anti-RAP2 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7974 dilution 10 ug/ml.
Species: Human, Monkey, Mouse, Dog, Horse, Opossum, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Anti-RAP2A Antibody (C-Term) at 1:2000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-RAP2A Antibody (N-Term) at 1:1000 dilution + mouse cerebellum lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human, Mouse
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

RAP2 antibody LS-C151601 is an unconjugated rabbit polyclonal antibody to RAP2 from human, mouse, rat and other species. Validated for WB.
About RAP2
Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading. P10114 NM_021033 NP_066361.1

RAP2A Antibody, K-REV Antibody, Ras-related protein rap-2b Antibody, RAP2 Antibody, Ras-related protein Rap-2a Antibody, RbBP-30 Antibody, KREV Antibody


Specifications

Target
Human RAP2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Drosophila (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
RAP2 antibody was raised against synthetic peptide located between aa31-80 of human RAP2A (P10114, NP_066361). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Salmon, Smelt, Zebrafish (100%); Drosophila (92%); Sea squirt, Beetle (85%).
Blocking Peptide
LS-E23349 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C151601. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa31-80
Specificity
Human RAP2A
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RAP2 antibody Western Blot of 721_B lysate. Antibody Dilution: 1.0 ug/ml. RAP2A is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells. Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAP2 antibody Western Blot of 721_B lysate. Antibody Dilution: 1.0 ug/ml. RAP2A is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells. Antibody dilution: 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RAP2 antibody Western Blot of HeLa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAP2 antibody Western Blot of HeLa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RAP2 antibody Western Blot of 721_B lysate. Antibody Dilution: 1.0 ug/ml. RAP2A is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells. Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAP2 antibody Western Blot of 721_B lysate. Antibody Dilution: 1.0 ug/ml. RAP2A is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells. Antibody dilution: 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RAP2 antibody Western Blot of HeLa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAP2 antibody Western Blot of HeLa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RAP2 antibody Western Blot of 721_B lysate. Antibody Dilution: 1.0 ug/ml. RAP2A is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells. Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAP2 antibody Western Blot of 721_B lysate. Antibody Dilution: 1.0 ug/ml. RAP2A is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells. Antibody dilution: 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RAP2 antibody Western Blot of HeLa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAP2 antibody Western Blot of HeLa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RAP2 antibody Western Blot of 721_B lysate. Antibody Dilution: 1.0 ug/ml. RAP2A is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells. Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAP2 antibody Western Blot of 721_B lysate. Antibody Dilution: 1.0 ug/ml. RAP2A is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells. Antibody dilution: 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RAP2 antibody Western Blot of HeLa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAP2 antibody Western Blot of HeLa. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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