Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
WB: Affinity Precipitation and Immunoblot Analysis: Representative blot from a previous lot. Samples were treated as described for affinity precipitation assay. Lane 1: HEK293 lysate in Rap1 Activation Lysis Buffer, 2X preincubated with GDP prior to precipitation with Ral GDS-RBD; Lane 2: HEK293 lysate in RLB preincubated with GTPÎ³S prior to precipitation with Ral GDS-RBD. Proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Rap1 (1Âµg/ml). Visualization was achieved using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Rap1 (~22kDa).