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Anti-RAP1A + RAP1B Antibody (aa31-48) LS-C152795

Catalog Size Price
LS-C152795-50 50 µg Unavailable

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5 RAP1A + RAP1B Antibodies

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Anti-RAP1A + RAP1B Antibody (N-Terminus) LS-C63168
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Anti-RAP1A + RAP1B Antibody (aa31-48) LS-C152795
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Western blot Image
Anti-RAP1A + RAP1B Antibody LS-C203009
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100% Guaranteed
Rabbit Polyclonal (IgG) to Human RAP1A + RAP1B
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken
Western blot
Unconjugated

Details

Human RAP1A + RAP1B
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Xenopus, Zebrafish, Drosophila (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

Western blot

Specificity and Use

RAP1A + RAP1B antibody was raised against kLH-conjugated, synthetic peptide (KYDPTIEDSYRKQVEVDC) corresponding to amino acids 31-48 human Rap1a Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Bat, Dog, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%); Opossum, Xenopus, Trout, Seabass, Catfish, Salmon, Smelt, Sablefish, Stickleback, Medaka, Pike, Pufferfish, Zebrafish, Drosophila, Slime mold (94%).
aa31-48
Recognizes recombinant human Rap1a and Rap1b proteins

Packaging

0.02 M phosphate buffer, pH 7.6, 0.25 M sodium chloride, 0.1% sodium azide
Store at 4°C.
For research use only.

About RAP1A + RAP1B


Western blot

Western blot
WB: Affinity Precipitation and Immunoblot Analysis: Representative blot from a previous lot. Samples were treated as described for affinity precipitation assay. Lane 1: HEK293 lysate in Rap1 Activation Lysis Buffer, 2X preincubated with GDP prior to precipitation with Ral GDS-RBD; Lane 2: HEK293 lysate in RLB preincubated with GTPγS prior to precipitation with Ral GDS-RBD. Proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Rap1 (1µg/ml). Visualization was achieved using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Rap1 (~22kDa).

Requested From: 
Date Requested: 6/22/2017

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