Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p.
IHC of paraffin-embedded Human liver tissue using anti-RANGAP1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RANGAP1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human prostate tissue using anti-RANGAP1 mouse monoclonal antibody. (Dilution 1:50).
Anti-RANGAP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RANGAP1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RANGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RANGAP1.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-RANGAP1 monoclonal antibody.
Flow cytometry of Jurkat cells, using anti-RANGAP1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of HeLa cells, using anti-RANGAP1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RANGAP1 antibody, and then analyzed by flow cytometry.
Requested From: United States
Date Requested: 2/24/2017