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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RALB Antibody (clone 4D1) IHC‑plus™ LS‑B5523
Note: This antibody replaces LS-C133078
RALB antibody LS-B5523 is an unconjugated mouse monoclonal antibody to human RALB. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5523-50
50 µg (0.5 mg/ml)
Unavailable

Popular RALB Products

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Product Description

RALB antibody LS-B5523 is an unconjugated mouse monoclonal antibody to human RALB. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About RALB
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. P11234 NM_002881 NP_002872.1

RALB Antibody, GTP binding protein) Antibody, RAS-like protein B Antibody, Ras-related protein Ral-B Antibody


Specifications

Target
Human RALB
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [4D1]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RALB antibody was raised against rALB (AAH18163, 89 a.a. ~ 183 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human RALB
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell line lysates and the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-RALB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5523 concentration 5 ug/ml.
Anti-RALB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5523 concentration 5 ug/ml.

Western blot

Western blot of RALB expression in transfected 293T cell line by RALB monoclonal antibody clone 4D1.
Western blot of RALB expression in transfected 293T cell line by RALB monoclonal antibody clone 4D1.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RALB is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RALB is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-RALB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5523 concentration 5 ug/ml.
Anti-RALB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5523 concentration 5 ug/ml.

Western blot

Western blot of RALB expression in transfected 293T cell line by RALB monoclonal antibody clone 4D1.
Western blot of RALB expression in transfected 293T cell line by RALB monoclonal antibody clone 4D1.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RALB is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RALB is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-RALB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5523 concentration 5 ug/ml.
Anti-RALB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5523 concentration 5 ug/ml.

Western blot

Western blot of RALB expression in transfected 293T cell line by RALB monoclonal antibody clone 4D1.
Western blot of RALB expression in transfected 293T cell line by RALB monoclonal antibody clone 4D1.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RALB is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RALB is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-RALB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5523 concentration 5 ug/ml.
Anti-RALB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5523 concentration 5 ug/ml.

Western blot

Western blot of RALB expression in transfected 293T cell line by RALB monoclonal antibody clone 4D1.
Western blot of RALB expression in transfected 293T cell line by RALB monoclonal antibody clone 4D1.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RALB is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RALB is approximately 0.3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/20/2018

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