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Anti-RAG2 / RAG-2 Antibody (clone 2G8) LS-C198057

Catalog Size Price
LS-C198057-100 100 µg Unavailable

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11 RAG2 / RAG-2 Antibodies

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Anti-RAG2 / RAG-2 Antibody (aa271-527) LS-C186092
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Anti-RAG2 / RAG-2 Antibody (aa498-527) LS-C408005
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Anti-RAG2 / RAG-2 Antibody LS-C482475
Rabbit Polyclonal to Human RAG2 / RAG-2
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ICC, Immunofluorescence, Western blot
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Immunofluorescent analysis of RAG-2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

100% Guaranteed
Mouse Monoclonal [clone 2G8] (IgG2b,k) to Human RAG2 / RAG-2
Human, Rat
Western blot, ELISA
Unconjugated

Details

Human RAG2 / RAG-2
Mouse
Human, Rat (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [2G8]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Western blot
  • ELISA

Specificity and Use

RAG2 / RAG-2 antibody was raised against rAG2 (NP_000527.1, aa428-527 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human RAG2 / RAG-2
Sandwich ELISA: Recombinant protein. Western Blot: Tissue lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About RAG2 / RAG-2

P55895 M94633 AAH22397.1

RAG2 Antibody, RAG-2 Antibody

Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments.

Western blot

RAG2 monoclonal antibody (M06), clone 2G8. Western Blot analysis of RAG2 expression in rat liver.
RAG2 monoclonal antibody (M06), clone 2G8. Western Blot analysis of RAG2 expression in rat liver.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RAG2 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RAG2 is approximately 1 ng/ml as a capture antibody.

Requested From: 
Date Requested: 4/23/2018

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