Target
Human RAF1 / RAF
Synonyms
RAF1 | C-Raf | C-raf-1 | CRAF | NS5 | Raf-1 | Proto-oncogene c-RAF | Oncogene RAF1 | RAF | Raf1 kinase
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Produced against a chemically synthesized phosphopeptide derived from a region of human c-Raf that contains tyrosines 340 and 341. The sequence is conserved in mouse and rat.
Specificity
Reactivity: Human c-Raf. Mouse and rat c-Raf (100% homologous) have not been tested, but are expected to react.
Usage
Suitable for use in Western blotting applications. Previous lots of this antibody have been used in immunocytochemistry. The current lot has not yet been validated for this application. For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. Positive control: HEK293 overexpressing c-Raf, in vitro-phosphorylated by active Src.
Presentation
1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee