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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAF1 / RAF Antibody (phospho‑Ser289) LS‑C198830
RAF antibody LS-C198830 is an unconjugated rabbit polyclonal antibody to RAF (RAF1) from human, mouse and rat. Validated for IHC and Peptide-ELISA.
Catalog
Size
Price
LS-C198830-50
50 µl
Unavailable
LS-C198830-100
100 µl
Unavailable

Popular RAF1 / RAF Products

Anti-RAF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1487 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry
Anti-RAF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6239 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
IHC of paraffin-embedded liver cancer tissues using RAF1 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry, ELISA

Product Description

RAF antibody LS-C198830 is an unconjugated rabbit polyclonal antibody to RAF (RAF1) from human, mouse and rat. Validated for IHC and Peptide-ELISA.
About RAF1 / RAF
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. P04049 NM_002880 NP_002871.1

RAF1 Antibody, C-Raf Antibody, C-raf-1 Antibody, CRAF Antibody, NS5 Antibody, Raf-1 Antibody, Proto-oncogene c-RAF Antibody, Oncogene RAF1 Antibody, RAF Antibody, Raf1 kinase Antibody


Specifications

Target
Human RAF1 / RAF
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RAF1 / RAF antibody was raised against synthetic peptide derived from human C-RAF around the phosphorylation site of Ser289.
Epitope
pSer289
Specificity
C-RAF (Phospho-Ser289) Antibody detects endogenous levels of C-RAF only when phosphorylated at serine289.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human brain, using C-RAF (Phospho-Ser289) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using C-RAF (Phospho-Ser289) Antibody. The sample on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain, using C-RAF (Phospho-Ser289) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using C-RAF (Phospho-Ser289) Antibody. The sample on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain, using C-RAF (Phospho-Ser289) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using C-RAF (Phospho-Ser289) Antibody. The sample on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain, using C-RAF (Phospho-Ser289) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using C-RAF (Phospho-Ser289) Antibody. The sample on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/23/2018

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