RAF antibody LS-C9877 is an unconjugated mouse monoclonal antibody to human RAF (RAF1). Validated for ELISA and WB.
(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot (1 µg/ml)
RAF1 / RAF antibody was raised against purified recombinant Raf-1 (75kD).
Recognizes Raf-1 N-terminal (aa1-240).
Suitable for use in ELISA and Western Blot. Not suitable for the detection of Raf-1 expression and phosphorylation in HeLa cells. Western Blot: 1 ug/ml will allow visualization of 0.2 ug/lane of human Raf-1. A main band (75kD) was visible.
PBS, pH 7.2, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.