RAF1 / RAF

v-raf-1 murine leukemia viral oncogene homolog 1

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

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Antibodies (242)

ELISA Kits (13)

Proteins (8)

Type

Cell-Based (1)

Cell-Based Phosphorylation (6)

Custom (4)

Over-Expression Lysate (2)

Primary (242)

Recombinant (6)

Sandwich (2)

Target

RAF1 / RAF (263)

Reactivity

Human (248)

Mouse (118)

Rat (106)

Bat (4)

Bovine (12)

Chicken (11)

Chimpanzee (3)

Dog (5)

Frog (6)

Gibbon (3)

Hamster (1)

Horse (7)

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Non-Human Primate (1)

Pig (4)

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Xenopus (4)

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IHC (91)

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Peptide-ELISA (24)

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rabbit (226)

mouse (14)

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IHCPlus (3)

Isotype

Ig (2)

IgG (178)

IgG1 (3)

IgG1,k (3)

IgG2b,k (2)

IgY (1)

Clonality

monoclonal mc (15)

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Format

96-Well Microplate (7)

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FITC Conjugated (20)

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Unconjugated (143)

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Epitope

pSer338 (18)

pSer259 (16)

pSer296 (12)

pSer43 (10)

pThr269 (9)

pTyr341 (9)

aa342-619 (8)

aa1-30 (7)

aa86-113 (7)

pSer339 (7)

pSer471 (7)

pSer494 (7)

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N-Terminus (6)

Ser494 (6)

Thr491 (6)

aa256-286 (6)

pSer621 (6)

aa1-648 (4)

aa241-290 (4)

pSer642 (4)

pSer301 (3)

200-280 aa (2)

240-320 aa (2)

280-360 aa (2)

30-110 aa (2)

C-Terminus (2)

pSer289 (2)

220-300 aa (1)

560-640 aa (1)

580-660 aa (1)

Arg282 (1)

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Internal (1)

Pro302 (1)

Ser291 (1)

Ser296 (1)

Ser338 (1)

aa11-60 (1)

aa231-280 (1)

aa233-282 (1)

aa251-300 (1)

aa266-315 (1)

aa271-320 (1)

aa286-335 (1)

aa305-354 (1)

aa311-360 (1)

aa598-648 (1)

aa657-668 (1)

p259 (1)

p289 (1)

p296 (1)

p301 (1)

p338 (1)

p341 (1)

p43 (1)

p621 (1)

pTyr340/Tyr341 (1)

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Publications

No (263)

Sample Type

Adherent Cell Cultures (7)

Cell Lysates (1)

Plasma (1)

Serum (1)

Tissue Homogenates (1)

Tag

GST (1)

GST, N-terminus (1)

His, N-Terminal (1)

His-SUMO, N-terminus (1)

His-T7 (1)

Myc-DDK (Flag) (3)

Species

Human (7)

Source

293T Cells (1)

E. coli (2)

HEK 293 Cells (2)

Sf9 Cells (1)

Wheat Germ Extract (2)

Purification

Purified (1)

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IHC-plus™ RAF1 / RAF Antibody (aa86-113) LS-B11821

(4.0) Rating

Antibody:

RAF1 / RAF Rabbit anti-Mouse Polyclonal (aa86-113) Antibody

Reactivity:

Mouse, Human

Applications:

Flo, IHC, IHC-P, WB

Format:

Unconjugated

Price:

200 µl/$460

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IHC-plus™ RAF1 / RAF Antibody (N-Terminus) LS-B6239

(4.0) Rating

Antibody:

RAF1 / RAF Mouse anti-Human Monoclonal (N-Terminus) Antibody

Reactivity:

Human

Applications:

ELISA, IHC, IHC-P, WB

Format:

Unconjugated

Price:

50 µg/$460

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RAF1 / RAF Antibody (aa1-30) LS-C97203

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (aa1-30) Antibody

Reactivity:

Human

Applications:

Flo, IF, IHC, WB

Format:

Unconjugated

Price:

400 µl/$393

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Human RAF1 / RAF Protein (Recombinant His-SUMO, N-terminus) (Full Length) - LS-G21115

Source:

E. coli

Tag:

His-SUMO, N-terminus

Molecular Weight:

89 kD

Price:

1 mg/$1,355; 100 µg/$420; 20 µg/$323

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Human RAF1 / RAF Protein (Recombinant GST) (Full Length) - LS-G30097

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

99.5 kDa

Price:

2 µg/$439

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IHC-plus™ RAF1 / RAF Antibody (C-Terminus) LS-B14835

(4.0) Rating

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Reactivity:

Chicken, Bovine, Mouse, Rat, Human

Applications:

ICC, IF, IHC, IHC-P, WB

Format:

Unconjugated

Price:

50 µl/$460

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RAF1 / RAF Antibody (clone 4G4) LS-C169472

Antibody:

RAF1 / RAF Mouse anti-Human Monoclonal (4G4) Antibody

Reactivity:

Human

Applications:

ELISA, Flo, IHC, IHC-P, WB

Format:

Unconjugated

Price:

100 µl/$459

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Human RAF1 / RAF Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G66803

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

72.9 kDa

Price:

20 µg/$1,107

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RAF1 / RAF Antibody (phospho-Ser621) LS-C800923

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (pSer621) Antibody

Reactivity:

Mouse, Rat, Human

Applications:

IF, IHC, Peptide-ELISA, WB

Format:

Unconjugated

Price:

100 µl/$379; 200 µl/$421

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Human RAF1 / RAF Protein (Recombinant His-T7) (aa342-619) - LS-G14881

Source:

E. coli

Tag:

His-T7

Molecular Weight:

35.6 kDa

Price:

10 µg/$271; 50 µg/$382; 100 µg/$610; 200 µg/$762; 1 mg/$1,817; 500 µg/$1,378; 5 mg/$2,775; 2 mg/$1,998

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Human RAF1 / RAF (Sandwich ELISA) ELISA Kit - LS-F7688

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Tissue Homogenates

Price:

1 Plate/$834

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RAF1 / RAF Antibody (phospho-Ser338) LS-C800926

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (pSer338) Antibody

Reactivity:

Mouse, Rat, Human

Applications:

IF, Peptide-ELISA, WB

Format:

Unconjugated

Price:

100 µl/$379; 200 µl/$421

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RAF1 / RAF Antibody (aa256-286, Biotin) LS-C281924

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (aa256-286) (Biotin) Antibody

Reactivity:

Human

Applications:

ELISA, IHC, WB

Format:

Biotin Conjugated

Price:

200 µl/$952

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RAF1 / RAF Antibody (Pro302) LS-C9879

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (Pro302) Antibody

Reactivity:

Human, Monkey

Applications:

IP, WB

Format:

Unconjugated

Price:

100 µl/$688

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RAF1 / RAF Antibody (phospho-Ser338) LS-C97056

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (pSer338) Antibody

Reactivity:

Human

Applications:

DB, WB

Format:

Unconjugated

Price:

400 µl/$393

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RAF1 / RAF Antibody (aa657-668) LS-C9887

Antibody:

RAF1 / RAF Sheep anti-Human Polyclonal (aa657-668) Antibody

Reactivity:

Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Horse, Bat, Human, Monkey

Applications:

IP, WB

Format:

Unconjugated

Price:

100 µg/$567

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RAF1 / RAF Antibody (clone 4G4) LS-C169173

Antibody:

RAF1 / RAF Mouse anti-Human Monoclonal (4G4) Antibody

Reactivity:

Human

Applications:

ELISA, Flo, IHC, WB

Format:

Unconjugated

Price:

100 µl/$459

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RAF1 / RAF Antibody (aa342-619) LS-C293553

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (aa342-619) Antibody

Reactivity:

Human

Applications:

WB

Format:

Unconjugated

Price:

100 µl/$309; 200 µl/$351; 500 µl/$491; 1 ml/$561; 2 ml/$755; 5 ml/$1,225

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RAF1 / RAF Antibody (phospho-Ser621) LS-C826836

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (pSer621) Antibody

Reactivity:

Mouse, Rat, Human

Applications:

ELISA, IF, IHC, WB

Format:

Unconjugated

Price:

100 µg/$357

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RAF1 / RAF Antibody (p621) LS-C826838

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (p621) Antibody

Reactivity:

Mouse, Rat, Human

Applications:

ELISA, IF, IHC, WB

Format:

Unconjugated

Price:

100 µg/$357

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RAF1 / RAF Antibody (phospho-Ser338) LS-C38712

Antibody:

RAF1 / RAF Rabbit anti-Human Monoclonal (pSer338) Antibody

Reactivity:

Mouse, Rat, Human, Monkey

Applications:

WB

Format:

Unconjugated

Price:

100 µl/$689

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RAF1 / RAF Antibody (phospho-Ser296) LS-C800922

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (pSer296) Antibody

Reactivity:

Mouse, Rat, Human

Applications:

IF, IHC, Peptide-ELISA, WB

Format:

Unconjugated

Price:

100 µl/$379; 200 µl/$421

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RAF1 / RAF Antibody (phospho-Ser259) LS-C800925

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (pSer259) Antibody

Reactivity:

Mouse, Rat, Human

Applications:

IF, IHC, Peptide-ELISA, WB

Format:

Unconjugated

Price:

100 µl/$379; 200 µl/$421

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RAF1 / RAF Antibody (aa86-113) LS-C330063

Antibody:

RAF1 / RAF Rabbit anti-Mouse Polyclonal (aa86-113) Antibody

Reactivity:

Mouse, Human

Applications:

ELISA, Flo, IHC, WB

Format:

Unconjugated

Price:

200 µl/$734

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RAF1 / RAF Antibody (phospho-Ser621) LS-C416444

Antibody:

RAF1 / RAF Rabbit anti-Human Polyclonal (pSer621) Antibody

Reactivity:

Mouse, Rat, Human

Applications:

ICC, IF, IHC, WB

Format:

Unconjugated

Price:

50 µg/$305; 100 µg/$357

Viewing 1-25 of 263 product results

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