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Anti-RAF1 / RAF Antibody (aa657-668) LS-C9887

Ordering

Wt. Vol. Conc. Price
100 µg - - $375
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RAF1 / RAF Antibodies

Anti-RAF1 / RAF Antibody (N-Terminus) IHC-plus™ LS-B6239
Mouse Monoclonal (IgG1,k) to Human RAF1 / RAF
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RAF1 / RAF Antibody (clone 4G4) LS-C169472
Mouse Monoclonal [clone 4G4] (IgG1) to Human RAF1 / RAF
Human
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human RAF1 / RAF
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Western blot, Immunoprecipitation
Unconjugated

Details

Human RAF1 / RAF
Sheep
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot
  • Immunoprecipitation

Specificity and Use

RAF1 / RAF antibody was raised against 12 residue synthetic C terminal peptide (CTLTTSPRLPVF) corresponding to residues 637-648 of human Raf-1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).
aa657-668
Recognizes Raf-1, Mr ~70kD. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat based on sequence homology.
Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 ug/ml detects Raf-1 in RIPA lysates from 3T3/A31 cell lysates. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Raf-1 (2 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Included Positive Antigen Control: 3T3/A31 cell lysate. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels. MAPK Activation and MBP Phosphorylation: 4 ug immunoprecipitates Raf-1. The immunocomplex was then used in a coupled phosphorylation assay to activate MEK1, which in turn activates MAP Kinase 2 that phosphorylates myelin basic protein in vitro.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About RAF1 / RAF

P04049 NM_002880 NP_002871.1

RAF1 Antibody, C-Raf Antibody, C-raf-1 Antibody, CRAF Antibody, NS5 Antibody, Raf-1 Antibody, Proto-oncogene c-RAF Antibody, Oncogene RAF1 Antibody, RAF Antibody, Raf1 kinase Antibody

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation.

Requested From: United States
Date Requested: 12/5/2016

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