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Antibodies > RAF1 / RAF > LS-C370255
 
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Catalog Number Size Price
LS-C370255-50 50 µg $294 
LS-C370255-100 100 µg $360 
RAF1 / RAF Antibody - IHC image of RAF1 Antibody diluted at 1:1200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
RAF1 / RAF Antibody - Immunofluorescence staining of Hela cells with RAF1 Antibody at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
RAF1 / RAF Antibody - Immunoprecipitating RAF1 in 293T whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of RAF1 Antibody in 293T whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: RAF1 Antibody (6µg) + 293T whole cell lysate (500µg) Lane 3: 293T whole cell lysate (10µg)
RAF1 / RAF Antibody - IHC image of RAF1 Antibody diluted at 1:1200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
RAF1 / RAF Antibody - Immunofluorescence staining of Hela cells with RAF1 Antibody at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
RAF1 / RAF Antibody - Immunoprecipitating RAF1 in 293T whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of RAF1 Antibody in 293T whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: RAF1 Antibody (6µg) + 293T whole cell lysate (500µg) Lane 3: 293T whole cell lysate (10µg)
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Polyclonal Rabbit anti‑Human RAF1 / RAF Antibody (aa1‑648) LS‑C370255

Polyclonal Rabbit anti‑Human RAF1 / RAF Antibody (aa1‑648) LS‑C370255

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Antibody:
RAF1 / RAF Rabbit anti-Human Polyclonal (aa1-648) Antibody
Application:
ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Biotin HRP FITC
Price
Catalog Number
$294
LS-C370255-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RAF1 / RAF Rabbit anti-Human Polyclonal (aa1-648) Antibody
Application:
ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Biotin HRP FITC

Specifications

Description
RAF antibody LS-C370255 is an unconjugated rabbit polyclonal antibody to human RAF (RAF1) (aa1-648). Validated for ELISA.
Target
Human RAF1 / RAF
Synonyms
RAF1 | C-Raf | C-raf-1 | CRAF | NS5 | Raf-1 | Proto-oncogene c-RAF | Oncogene RAF1 | RAF | Raf1 kinase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Recombinant human RAF proto-oncogene serine/threonine-protein kinase protein (aa1-648).
Epitope
aa1-648
Specificity
Human RAF1 / RAF
Applications
  • ELISA
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAF1 / RAF
P04049 NM_002880 NP_002871.1

Publications (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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