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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAD9A / RAD9 Antibody (clone 7B11) LS‑C114537
RAD9 antibody LS-C114537 is an unconjugated mouse monoclonal antibody to RAD9 (RAD9A). Validated for IHC and WB.
Catalog
Size
Price
LS-C114537-100
100 µl
Unavailable

Popular RAD9A / RAD9 Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Rad9 antibody detects RAD9A protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. Rad9 antibody dilution:1:500.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Immunohistochemistry - Paraffin Image
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot

Product Description

RAD9 antibody LS-C114537 is an unconjugated mouse monoclonal antibody to RAD9 (RAD9A). Validated for IHC and WB.
About RAD9A / RAD9
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). Q99638 NM_004584 NP_004575.1

RAD9A Antibody, HRAD9 Antibody, RAD9 Antibody, RAD9 homolog Antibody, RAD9 (S. pombe) homolog Antibody, RAD9 homolog A (S. pombe) Antibody


Specifications

Target
Human RAD9A / RAD9
Host
Mouse
Clonality
IgG2a Monoclonal [7B11]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RAD9A / RAD9 antibody was raised against protein expressed in 293T cell transfected with human RAD9A expression vector
Specificity
Human RAD9A
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD9A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD9A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD9A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD9A.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-RAD9Amonoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-RAD9Amonoclonal antibody.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD9A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD9A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD9A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD9A.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-RAD9Amonoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-RAD9Amonoclonal antibody.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD9A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD9A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD9A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD9A.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-RAD9Amonoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-RAD9Amonoclonal antibody.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded colon tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RAD9A mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD9A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD9A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD9A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD9A.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-RAD9Amonoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-RAD9Amonoclonal antibody.


Requested From: 
Date Requested: 6/23/2018

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