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Antibodies > RAD51 / RECA > LS-C357511
 
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Catalog Number Size Price
LS-C357511-10 10 µg $318 
LS-C357511-100 100 µg (0.5 mg/ml) $470 
RAD51 / RECA Antibody - Rad51 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
RAD51 / RECA Antibody - Rad51 antibody IHC-paraffin: Rat Intestine Tissue.
RAD51 / RECA Antibody - Western blot analysis of Rad51 using anti-Rad51 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testicular tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testicular tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: mouse RAW246. 7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 37KD.
RAD51 / RECA Antibody - Western blot analysis of Rad51 using anti-Rad51 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human HL-60 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 37KD.
RAD51 / RECA Antibody - Rad51 antibody Western blot. All lanes: Anti Rad51 at 0.5 ug/ml. Lane 1: 22RV1 Whole Cell Lysate at 40 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Lane 4: U87 Whole Cell Lysate at 40 ug. Lane 5: CEM Whole Cell Lysate at 40 ug. Lane 6: MM231 Whole Cell Lysate at 40 ug. Predicted band size: 37 kD. Observed band size: 37 kD.
RAD51 / RECA Antibody - Rad51 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
RAD51 / RECA Antibody - Rad51 antibody IHC-paraffin: Rat Intestine Tissue.
RAD51 / RECA Antibody - Western blot analysis of Rad51 using anti-Rad51 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testicular tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testicular tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: mouse RAW246. 7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 37KD.
RAD51 / RECA Antibody - Western blot analysis of Rad51 using anti-Rad51 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human HL-60 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 37KD.
RAD51 / RECA Antibody - Rad51 antibody Western blot. All lanes: Anti Rad51 at 0.5 ug/ml. Lane 1: 22RV1 Whole Cell Lysate at 40 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Lane 4: U87 Whole Cell Lysate at 40 ug. Lane 5: CEM Whole Cell Lysate at 40 ug. Lane 6: MM231 Whole Cell Lysate at 40 ug. Predicted band size: 37 kD. Observed band size: 37 kD.
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Polyclonal Rabbit anti‑Human RAD51 / RECA Antibody (aa1‑258, IHC, WB) LS‑C357511

Polyclonal Rabbit anti‑Human RAD51 / RECA Antibody (aa1‑258, IHC, WB) LS‑C357511

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Note: This antibody replaces LS-C389176
Antibody:
RAD51 / RECA Rabbit anti-Human Polyclonal (aa1-258) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357511-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RAD51 / RECA Rabbit anti-Human Polyclonal (aa1-258) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
RECA antibody LS-C357511 is an unconjugated rabbit polyclonal antibody to RECA (RAD51) (aa1-258) from human. It is reactive with human and rat. Validated for IHC and WB.
Target
Human RAD51 / RECA
Synonyms
RAD51 | BRCC5 | HsT16930 | RAD51 homolog A | RAD51 homolog (S. cerevisiae) | RecA, E. coli, homolog of | RecA-like protein | Recombination protein A | MRMV2 | HRAD51 | HsRad51 | RAD51A | RECA
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Rad51 recombinant protein (Position: M1-E258). Human Rad51 shares 98% amino acid (aa) sequence identity with mouse Rad51.
Epitope
aa1-258
Specificity
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAD51 / RECA
Q06609 NM_002875 NP_002866.2

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RAD51 / RECA Antibody - Anti-RAD51 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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