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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAD50 Antibody (aa1‑425) LS‑C17557
RAD50 antibody LS-C17557 is an unconjugated mouse monoclonal antibody to human RAD50. Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C17557-100
100 µg
$635

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Product Description

RAD50 antibody LS-C17557 is an unconjugated mouse monoclonal antibody to human RAD50. Validated for IP and WB.

Specifications

Target
Human RAD50
Synonyms
RAD50, DNA repair protein RAD50, HRad50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
RAD50 antibody was raised against gST fusion protein corresponding to residues 1-425 of human RAD50. The immunogen shares 389/425 identity with mouse and 127/425 identity with yeast.
Epitope
aa1-425
Specificity
Recognizes human Rad50.
Usage
Western Blot Analysis: 0.5-2 ug/ml detects RAD50 in a HeLa nuclear lysate. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Rad50 (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: HeLa nuclear lysate. Use 20 ug per lane for minigels. Immunoprecipitation: Reported to immunoprecipitate.
Presentation
10 mM PBS, pH 7.4
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About RAD50
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. Q92878 NM_005732 NP_005723.2


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Requested From: United States
Date Requested: 10/19/2018

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