Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
RAD50 antibody was raised against gST fusion protein corresponding to residues 1-425 of human RAD50. The immunogen shares 389/425 identity with mouse and 127/425 identity with yeast.
Recognizes human Rad50.
Western Blot Analysis: 0.5-2 ug/ml detects RAD50 in a HeLa nuclear lysate. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Rad50 (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: HeLa nuclear lysate. Use 20 ug per lane for minigels. Immunoprecipitation: Reported to immunoprecipitate.
10 mM PBS, pH 7.4
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity.