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Anti-RAD50 Antibody (aa1-425, clone 13B3) IHC-plus™ LS-B1708

Note: This antibody replaces LS-C20035

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RAD50 Antibodies

Anti-RAD50 Antibody (aa1-425, clone 4H9) LS-C53371
Mouse Monoclonal [clone 4H9] (IgG1) to Human RAD50
Human
Western blot, Immunoprecipitation
Unconjugated
Anti-RAD50 Antibody (aa628-787) LS-C93574
Rabbit Polyclonal to Human RAD50
Human
Western blot, Immunoprecipitation
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 13B3] (IgG1) to Human RAD50
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Human RAD50
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [13B3]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation

Specificity and Use

RAD50 antibody was raised against recombinant human RAD50.
aa1-425
Amino acids 1-425 of Rad50 expressed in E. coli.
Immunohistochemistry: LS-B1708 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1708 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.4. No preservative added.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About RAD50

Q92878 NM_005732 NP_005723.2

RAD50 Antibody, DNA repair protein RAD50 Antibody, HRad50 Antibody, NBSLD Antibody, RAD50 (S. cerevisiae) homolog Antibody, RAD50 homolog (S. cerevisiae) Antibody, RAD502 Antibody, RAD50-2 Antibody

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity.

Immunohistochemistry

Immunohistochemistry
Anti-RAD50 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1708 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/9/2016

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