Human RAD23B / HR23B

Goat

Human (tested or 100% immunogen sequence identity)

Chimpanzee, Dog, S. cerevisiae (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 155-180 of human HR23B protein.

aa155-180

Reactivity occurs against human HR23B protein. Sequence homology as assessed by BLAST indicated 100% homology for this protein from human, dog, chimpanzee and S. cerevisiae. Cross reactivity with HR23B protein from mouse and rat may also occur as sequence homology varies by one amino acid residue in this sequence by BLAST analysis. Reactivity with HR23B protein from other sources is not known.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (1:500 - 1:2000)
• ELISA (1:2000 - 1:10000)

Immunohistochemistry: LS-B412 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B412 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P54727 NCBI: NM_002874 NP_002865.1