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| Catalog Number | Size | Price |
|---|---|---|
| LS-B82-0.1 | 0.1 ml (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human RAD21 Antibody (clone CM110‑2C10, aa544‑631, IHC, WB) LS‑B82
IHC‑plus™ Monoclonal Mouse anti‑Human RAD21 Antibody (clone CM110‑2C10, aa544‑631, IHC, WB) LS‑B82
Note: This antibody replaces LS-C2637
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
RAD21 antibody LS-B82 is an unconjugated mouse monoclonal antibody to RAD21 (aa544-631) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RAD21
Synonyms
RAD21 | CDLS4 | HR21 | KIAA0078 | HHR21 | NXP-1 | NXP1 | RAD21 (S. pombe) homolog | MCD1 | Nuclear matrix protein 1 | RAD21 homolog (S. pombe) | HRAD21 | SCC1 | SCC1 homolog
Host
Mouse
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
CM110-2C10
Purification
Protein G purified
Modifications
Unmodified
Immunogen
An N-Terminus fusion protein of the C-Terminus fragment of Scc1 subunit human cohesin (aa 544-631 of hRad21).
Epitope
aa544-631
Specificity
both the nuclear hRad21 protein and the cleaved cytoplasmic hRad21 protein.
Applications
- IHC
- IHC - Paraffin (20 µg/ml)
- Western blot (1:500)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B82 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B82 was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About RAD21
Validation
Anti-RAD21 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RAD21 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ RAD21 Antibody (aa544-631, clone CM110-2C10) for IHC, WB/Western LS-B82 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
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Species:
Human, Monkey, Bovine, Guinea pig, Hamster, Pig
Applications:
IHC, Western blot
Reactivity:
Human
Range:
0.78-50 ng/ml
Reactivity:
Mouse
Range:
62.5-4000 pg/ml
Source:
E. coli
Tag:
His
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
