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Anti-RAD18 Antibody (clone 3H7) LS-C133790

Catalog Size Price
LS-C133790-100 100 µg (0.5 mg/ml) Unavailable

Related Products

26 RAD18 Antibodies

Most Popular RAD18 Antibodies

Anti-RAD18 Antibody (clone 3H7) LS-C133790
Mouse Monoclonal [clone 3H7] (IgG2b,k) to Human RAD18
Human, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RAD18 Antibody LS-C334025
Rabbit Polyclonal (IgG) to Human RAD18
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image
Anti-RAD18 Antibody (aa1-495, FITC) LS-C372267
Rabbit Polyclonal (IgG) to Human RAD18
Human
IHC, IHC - Paraffin, Western blot, ELISA
FITC Conjugated
Immunohistochemistry Image
Anti-RAD18 Antibody (aa1-495, Biotin) LS-C372268
Rabbit Polyclonal (IgG) to Human RAD18
Human
IHC, IHC - Paraffin, Western blot, ELISA
Biotin Conjugated
Immunohistochemistry Image
Anti-RAD18 Antibody (aa1-495, HRP) LS-C372270
Rabbit Polyclonal (IgG) to Human RAD18
Human
IHC, IHC - Paraffin, Western blot, ELISA
HRP Conjugated
Immunohistochemistry Image

100% Guaranteed
Mouse Monoclonal [clone 3H7] (IgG2b,k) to Human RAD18
Human, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human RAD18
Mouse
Human, Rat (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [3H7]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • IHC - Paraffin (1.5 µg/ml)
  • Immunofluorescence (25 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

RAD18 antibody was raised against rAD18 (NP_064550, 332 a.a. ~ 431 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human RAD18
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4.
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About RAD18

NM_020165

RAD18 Antibody, HHR18 Antibody, HRAD18 Antibody, RNF73 Antibody, RAD18 homolog (S. cerevisiae) Antibody, RAD18, S. cerevisiae, homolog Antibody, RING finger protein 73 Antibody

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.

Immunohistochemistry

Immunohistochemistry
Immunoperoxidase of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml]

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to RAD18 on HeLa cell. [antibody concentration 25 ug/ml]

Western blot

Western blot
RAD18 monoclonal antibody (M01), clone 3H7. Western blot of RAD18 expression in PC-12.

Western blot

Western blot
RAD18 monoclonal antibody (M01), clone 3H7 Western blot of RAD18 expression in HeLa NE.

Western blot

Western blot
Western blot of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RAD18 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody, clone 3H7. GAPDH ( 36.1 kD ) used as specificity.

Requested From: 
Date Requested: 10/21/2017

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