Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human RACGAP1 / MGCRACGAP
IHC - Paraffin, ICC, Western blot
Human RACGAP1 / MGCRACGAP
Human, Mouse (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000)
ICC (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
RACGAP1 / MGCRACGAP antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 202 of RACGAP1 (SwissProt Q9H0H5). Percent identity by BLAST analysis: Human (100%); Rat (84%).
Human RACGAP1 / MGCRACGAP
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
RACGAP1 Antibody, GTPase activating protein Antibody, ID-GAP Antibody, KIAA1478 Antibody, MgcRacGAP Antibody, Protein CYK4 homolg Antibody, Male germ cell RacGap Antibody, CYK4 Antibody, HsCYK-4 Antibody
Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity.
IHC of paraffin-embedded Hepatoma using RACGAP1 antibody at 1:500 dilution.
IHC of paraffin-embedded H1299 xenograft using RACGAP1 antibody at 1:500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431 using RACGAP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
Sample (30 ug of whole cell lysate). A: A431 , B: Raji. 7.5% SDS PAGE. RACGAP1 / MGCRACGAP antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: mouse heart. 7.5% SDS PAGE. RACGAP1 / MGCRACGAP antibody diluted at 1:1000.
Requested From: United States
Date Requested: 2/24/2017