(tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
Protein A purified
RAC1 antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser71 of human Rac1 and cdc42.
Detects endogenous Rac1 or cdc42 only when phosphorylated at Ser71. May also recognize phospho-RhoA (Ser73). Species cross-reactivity: Predicted to cross-react with mouse based on sequence homology.
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.