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Anti-RAC1 Antibody IHC-plus™ LS-B6306

Note: This antibody replaces LS-C9852

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RAC1 Antibodies

Anti-RAC1 Antibody IHC-plus™ LS-B6306
Mouse Monoclonal (IgG2b) to Human RAC1
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-RAC1 Antibody (clone 13A216, FITC) LS-C183408
Mouse Monoclonal [clone 13A216] (IgG2b) to Human RAC1
Human
Flow Cytometry
FITC Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2b) to Human RAC1
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation (applications tested for the base form of this product only)
Unconjugated

Details

Human RAC1
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG2b Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)

Specificity and Use

RAC1 antibody was raised against recombinant protein containing the full length human Rac1.
Recognizes human Rac1 at 21kD. Slight Rac2 cross-reactivity is observed. An additional unknown protein of ~55kD may be detected in some preparations. Species cross-reactivity: mouse and rat.
Suitable for use in Western Blot, Immunohistochemistry, Affinity Precipitation and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Proteins were detected by Western Blot analysis using 1 ug/ml. Immunoprecipitation: 4 ug immunoprecipitated Rac1 from a rat brain microsomal protein preparation.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About RAC1

P63000 NM_006908 NP_061485.1

RAC1 Antibody, MIG5 Antibody, p21-Rac1 Antibody, Ras-like protein TC25 Antibody, TC25 Antibody, TC-25 Antibody, Rac-1 Antibody

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Immunohistochemistry

Immunohistochemistry
Anti-RAC1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6306 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 2/27/2017

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