Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
RAB5A, Ras-related protein Rab-5A, RAS-associated protein RAB5A, RAB5
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster
(tested or 100% immunogen sequence identity)
RAB5A / RAB5 antibody was raised against human Rab5 synthetic peptide (aa182-197), conjugated to KLH. This sequence is identical to canine Rab5 over these residues.
Recognizes human Rab5 at ~26kD. An additional higher molecular mass band of unknown identity at ~45kD is detected in some samples on Western blots. Species cross-reactivity: Mouse, rat, bovine, canine, guinea pig, hamster and monkey.
Suitable for use in Western Blot, and Immunoprecipitation. Western Blot (ECL): 1:2000 dilution detected Rab5 in 20 ug of mouse brain tissue extract by ECL. Immunoprecipitation: 1:100. Positive control: Mouse Brain Tissue Extract, Rat Brain Tissue Extract.
PBS, 0.09% Sodium Azide, 50% Glycerol
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes.