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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAB5A / RAB5 Antibody (aa182‑197) LS‑C17543
RAB5 antibody LS-C17543 is an unconjugated rabbit polyclonal antibody to RAB5 (RAB5A) from human, mouse, rat and other species. Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C17543-25
25 µg
$305
LS-C17543-100
100 µg
$445

Popular RAB5A / RAB5 Products

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Mouse, Human, Monkey, Rat, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Immunofluorescence. Anti-Rab5a antibody in transduced Hepa1-6 cells with Ad GFP-Rab5a at 1:50 dilution. Cells were fixed with 4% of PFA.
Species: Mouse, Human, Monkey, Rat, Dog
Applications: Immunofluorescence, Western blot
Antibody
Species: Mouse, Rat
Applications: IHC, Western blot
Antibody
Species: Mouse, Rat
Applications: IHC, Western blot
Anti-RAB5A / RAB5 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

RAB5 antibody LS-C17543 is an unconjugated rabbit polyclonal antibody to RAB5 (RAB5A) from human, mouse, rat and other species. Validated for IP and WB.

Specifications

Target
Human RAB5A / RAB5
Synonyms
RAB5A, Ras-related protein Rab-5A, RAS-associated protein RAB5A, RAB5
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation (1:100)
Immunogen
RAB5A / RAB5 antibody was raised against human Rab5 synthetic peptide (aa182-197), conjugated to KLH. This sequence is identical to canine Rab5 over these residues.
Epitope
aa182-197
Specificity
Recognizes human Rab5 at ~26kD. An additional higher molecular mass band of unknown identity at ~45kD is detected in some samples on Western blots. Species cross-reactivity: Mouse, rat, bovine, canine, guinea pig, hamster and monkey.
Usage
Suitable for use in Western Blot, and Immunoprecipitation. Western Blot (ECL): 1:2000 dilution detected Rab5 in 20 ug of mouse brain tissue extract by ECL. Immunoprecipitation: 1:100. Positive control: Mouse Brain Tissue Extract, Rat Brain Tissue Extract.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About RAB5A / RAB5
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. P20339 NM_004162 NP_004153.2


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Requested From: United States
Date Requested: 10/21/2018

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