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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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RAB5A / RAB5 Antibody (aa107‑156) LS‑C107583
RAB5 antibody LS-C107583 is an unconjugated rabbit polyclonal antibody to RAB5 (RAB5A) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C107583-50
50 µg
Unavailable

Popular RAB5A / RAB5 Products

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Product Description

RAB5 antibody LS-C107583 is an unconjugated rabbit polyclonal antibody to RAB5 (RAB5A) from human, mouse, rat and other species. Validated for WB.
About RAB5A / RAB5
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. P20339 NM_004162 NP_004153.2

RAB5A Antibody, Ras-related protein Rab-5A Antibody, RAS-associated protein RAB5A Antibody, RAB5 Antibody


Specifications

Target
Human RAB5A / RAB5
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Predicted
Guinea pig, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
RAB5A / RAB5 antibody was raised against synthetic peptide located between aa107-156 of human RAB5A (P20339, NP_004153). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Platypus (100%); Guinea pig, Turkey, Zebra finch, Chicken, Lizard, Xenopus, Catfish, Salmon, Smelt, Stickleback, Zebrafish (92%); Orangutan (85%).
Blocking Peptide
LS-E20555 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C107583. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa107-156
Specificity
Human RAB5A / RAB5
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RAB5A / RAB5 antibody LS-C107583 Western blot of Fetal Muscle lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAB5A / RAB5 antibody LS-C107583 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RAB5A / RAB5 antibody LS-C107583 Western blot of Fetal Muscle lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAB5A / RAB5 antibody LS-C107583 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RAB5A / RAB5 antibody LS-C107583 Western blot of Fetal Muscle lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAB5A / RAB5 antibody LS-C107583 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RAB5A / RAB5 antibody LS-C107583 Western blot of Fetal Muscle lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RAB5A / RAB5 antibody LS-C107583 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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