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Anti-RAB3A Antibody (aa202-217) IHC-plus™ LS-B3406

Note: This antibody replaces LS-C63166, LS-C17534

Ordering

Wt. Vol. Conc. Price
50 µg - 0.85 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RAB3A Antibodies

Anti-RAB3A Antibody (aa202-217) IHC-plus™ LS-B3406
Rabbit Polyclonal to Human RAB3A
Human, Monkey
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RAB3A Antibody (aa1-30, APC) LS-C267709
Rabbit Polyclonal (IgG) to Human RAB3A
Human
Western blot, ELISA
APC Conjugated
Anti-RAB3A Antibody (clone 1531CT562.14.57) LS-C392563
Mouse Monoclonal [clone 1531CT562.14.57] (IgG1,k) to Human RAB3A
Human, Mouse, Rat
Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human RAB3A
Human, Monkey
IHC - Paraffin, Western blot
Unconjugated

Details

Human RAB3A
Rabbit
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 2 µg/ml)

Specificity and Use

RAB3A antibody was raised against a 16 residue synthetic peptide (C)KQGPQLSDQQVPPHQD based on the human Rab3A (residues 202-217) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).
aa202-217
Detects an ~25 kD protein, corresponding to the apparent molecular mass of Rab3A on SDSPAGE immunoblots, in samples from mouse and rat origins. The antibody specificity is confirmed in peptide inhibition immunoblotting studies.
Immunohistochemistry: LS-B3406 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3406 was determined to be 10 ug/ml.

Packaging

Borate buffered saline, pH 8.2 (25mM sodium borate, 100mM boric acid, 75mM NaCl, 5mM EDTA (disodium salt)
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About RAB3A

P20336 NM_002866 NP_002857.1

RAB3A Antibody, RAS-associated protein RAB3A Antibody, Ras-related protein Rab-3A Antibody

Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.

Immunohistochemistry

Immunohistochemistry
Anti-RAB3A antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3406 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-RAB3A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3406 concentration 10 ug/ml.

Requested From: United States
Date Requested: 12/7/2016

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