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Anti-RAB27A / RAB27 Antibody (clone 1G7) LS-C133077

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RAB27A / RAB27 Antibodies

Anti-RAB27A / RAB27 Antibody LS-C194031
Rabbit Polyclonal (IgG) to Human RAB27A / RAB27
Human, Mouse, Rat
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1G7] (IgG1,k) to Human RAB27A / RAB27
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human RAB27A / RAB27
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1G7]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (3 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

RAB27A / RAB27 antibody was raised against rAB27A (NP_004571, 122 a.a. ~ 222 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human RAB27A
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About RAB27A / RAB27

P51159 NM_004580 NP_004571.2

RAB27A Antibody, GS2 Antibody, GTP-binding protein Ram Antibody, HsT18676 Antibody, RAB27 Antibody, Ras-related protein Rab-27A Antibody, Rab-27 Antibody, RAM Antibody

RAB27A / RAB27 belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Immunohistochemistry

Immunohistochemistry
Immunoperoxidase of monoclonal antibody to RAB27A on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml]

Western blot

Western blot
RAB27A monoclonal antibody (M02), clone 1G7 Western blot of RAB27A expression in HL-60.

Western blot

Western blot
Western blot of RAB27A expression in transfected 293T cell line by RAB27A monoclonal antibody (M02), clone 1G7.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RAB27A is approximately 0.03 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of RAB27A over-expressed 293 cell line, cotransfected with RAB27A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAB27A monoclonal antibody, clone 1G7. GAPDH ( 36.1 kD ) used as specific.
 

Requested From: United States
Date Requested: 12/2/2016

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