Human RAB11FIP3

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids spanning the carboxyl terminal one-third of human FIP3.

A BLAST analysis was used to suggest cross-reactivity with Rab11 protein from mouse based on approximately 92% protein:protein homology over the region of the immunizing sequence. Reactivity against homologues from other sources is not known.

• IHC
• Western blot (1:200 - 1:2000)
• ELISA (1:2000 - 1:10000)

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Although the predicted molecular weight of FIP3 is 82.4 kD, a band corresponding to FIP3 was observed at 120 kD in human cell lysates and at 150 kD in mouse cell lysates. The identity of a 40 kD band, observed in western blotting against both HeLa and 293T cell lysates, is unknown (personal communication, P. Randazzo, CCR-NCI, Bethesda, MD).

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O75154 NCBI: NM_014700 NP_055515.1