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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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R1 / RRM1 Antibody LS‑C155176
RRM1 antibody LS-C155176 is an unconjugated rabbit polyclonal antibody to human RRM1 (R1). Validated for IHC and WB.
Catalog
Size
Price
LS-C155176-100
100 µl (1 mg/ml)
Unavailable

Popular R1 / RRM1 Products

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Applications: Western blot
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RRM1 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-R1 / RRM1 antibody IHC staining of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry of paraffin-embedded human prostate.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemical analysis of RRM1 staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

RRM1 antibody LS-C155176 is an unconjugated rabbit polyclonal antibody to human RRM1 (R1). Validated for IHC and WB.
About R1 / RRM1
R1 / RRM1 is one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. P23921 NM_001033 NP_001024.1

RRM1 Antibody, R1 Antibody, Ribonucleotide reductase M1 Antibody, RR1 Antibody, RIR1 Antibody


Specifications

Target
Human R1 / RRM1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
R1 / RRM1 antibody was raised against recombinant fragment corresponding to a region within amino acids 246 and 792 of RRM1 (SwissProt P23921). Percent identity by BLAST analysis: Human (100%); Mouse, Rat (97%); Bovine (95%); Chicken (92%).
Specificity
Human RRM1
Usage
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Storage
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Colon ca, using RRM1 antibody at 1:500 dilution.
IHC of paraffin-embedded Colon ca, using RRM1 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE RRM1 antibody diluted at 1:2000
Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE RRM1 antibody diluted at 1:2000

Immunohistochemistry

IHC of paraffin-embedded Colon ca, using RRM1 antibody at 1:500 dilution.
IHC of paraffin-embedded Colon ca, using RRM1 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE RRM1 antibody diluted at 1:2000
Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE RRM1 antibody diluted at 1:2000

Immunohistochemistry

IHC of paraffin-embedded Colon ca, using RRM1 antibody at 1:500 dilution.
IHC of paraffin-embedded Colon ca, using RRM1 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE RRM1 antibody diluted at 1:2000
Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE RRM1 antibody diluted at 1:2000

Immunohistochemistry

IHC of paraffin-embedded Colon ca, using RRM1 antibody at 1:500 dilution.
IHC of paraffin-embedded Colon ca, using RRM1 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE RRM1 antibody diluted at 1:2000
Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE RRM1 antibody diluted at 1:2000


Requested From: 
Date Requested: 6/19/2018

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