Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 6A8.6F10.1A6] (IgG1,k) to Mouse Qa-1b
Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [6A8.6F10.1A6]
Protein G purified
Flow Cytometry (0.5 - 1 µg/10E6 cells)
Specificity and Use
Qa-1b antibody was raised against animals were immunized with a synthetic peptide from the alpha-2 domain of Qa-1b. Qa-1 is a Class Ib molecule recognized by cytotoxic T cells and natural killer cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with the Qa-1b Class Ib molecule.
Cell surface staining of resting and ConA activated (5 ug/ml for 72 hr) BALB/C splenocytes using 0.5 ug of Qa-1b biotinylated antibody (LS-C148420). CD3 positive cells were gated. Streptavidin - PE (SA-PE) was used as secondary antibody. a) SA-PE resting cells. b) SA-PE ConA activated cells. c) Qa-1b resting cells. d) Qa-1b ConA activated cells.
Requested From: United States
Date Requested: 10/22/2016