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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PXN / Paxillin Antibody LS‑C131322
Antibody
Paxillin antibody LS-C131322 is an unconjugated rabbit polyclonal antibody to Paxillin (PXN) from human, bovine, monkey and other species. Validated for IF and WB.
Catalog
Size
Price
LS-C131322-50
50 µg
Unavailable

Popular PXN / Paxillin Products

Antibody
Species: Human, Mouse, Rat
Applications: Western blot
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PXN Antibody (Y118) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
IHC of paraffin-embedded human breast carcinoma tissue using Rabbit Anti-Paxillin (Ab-118) Polyclonal Antibody LS-B4259
Species: Human, Monkey, Mouse, Rat, Bovine, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Anti-Paxillin antibody IHC of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8618 dilution 10 ug/ml.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemical analysis of Paxillin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation

Product Description

Paxillin antibody LS-C131322 is an unconjugated rabbit polyclonal antibody to Paxillin (PXN) from human, bovine, monkey and other species. Validated for IF and WB.
About PXN / Paxillin
PXN / Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). P49023 NM_002859 NP_002850.2

PXN Antibody, Paxillin Antibody


Specifications

Target
Human PXN / Paxillin
Host
Rabbit
Reactivity
Human, Monkey, Bovine, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:500 - 1:1000)
Immunogen
PXN / Paxillin antibody was raised against synthetic non-phosphopeptide corresponding to human Paxillin around the phosphorylation site of tyrosine 88 (P-V-YP-G-S).
Specificity
Recognizes human Paxillin.
Usage
Suitable for use in Immunofluorescence and Western Blot. Immunofluorescence:1:100-1:200. Western Blot:1:500-1:1000.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/18/2018

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