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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PTPRT Antibody (aa58‑69) IHC‑plus™ LS‑B2604 Best Seller
Note: This antibody replaces LS-C55346
PTPRT antibody LS-B2604 is an unconjugated goat polyclonal antibody to human PTPRT. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2604-50
50 µg (0.5 mg/ml)
$425

Popular PTPRT Products

Immunohistochemistry of Human ovarian cancer using PTPRT Polyclonal Antibody at dilution of 1:30.
Species: Human, Mouse
Applications: IHC, ELISA
Western blot Image
Species: Human
Applications: Western blot, ELISA
PTPRT antibody LS-C146249 Western blot of COL0205 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Bat, Dog, Guinea pig, Horse, Rabbit, Zebrafish
Applications: Western blot
PTPRT antibody LS-C146249 Western blot of COL0205 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Bat, Dog, Guinea pig, Horse, Rabbit, Zebrafish
Applications: Western blot

Product Description

PTPRT antibody LS-B2604 is an unconjugated goat polyclonal antibody to human PTPRT. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About PTPRT
PTPRT is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. O14522 NM_007050 NP_008981.4

PTPRT Antibody, KIAA0283 Antibody, R-PTP-T Antibody, RPTP-rho Antibody, RPTPrho Antibody


Specifications

Target
Human PTPRT
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Hamster, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PTPRT antibody was raised against synthetic peptide C-NTWEKPMLDQAV from an internal region of human PTPRT (NP_573400.2; NP_008981.3). Percent identity with other species by BLAST analysis: Human (100%), Mouse (92%), Hamster (92%), Panda (92%), Rabbit (92%), Opossum (92%), Bat (83%).
Blocking Peptide
LS-E25771 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2604. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa58-69
Specificity
Human PTPRT. This antibody is expected to recognise both reported isoforms. Epitope corresponding to aa 58-69 of human PTPRT protein.
Usage
Immunohistochemistry: LS-B2604 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2604 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-PTPRT antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2604 concentration 5 ug/ml.
Anti-PTPRT antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2604 concentration 5 ug/ml.

Western blot

Antibody staining (0.3 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-PTPRT antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2604 concentration 5 ug/ml.
Anti-PTPRT antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2604 concentration 5 ug/ml.

Western blot

Antibody staining (0.3 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-PTPRT antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2604 concentration 5 ug/ml.
Anti-PTPRT antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2604 concentration 5 ug/ml.

Western blot

Antibody staining (0.3 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-PTPRT antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2604 concentration 5 ug/ml.
Anti-PTPRT antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2604 concentration 5 ug/ml.

Western blot

Antibody staining (0.3 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Requested From: United States
Date Requested: 9/25/2018

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