(tested or 100% immunogen sequence identity)
Mouse, Hamster, Rabbit (at least 90% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
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including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
PTPRT antibody was raised against synthetic peptide C-NTWEKPMLDQAV from an internal region of human PTPRT (NP_573400.2; NP_008981.3). Percent identity with other species by BLAST analysis: Human (100%), Mouse (92%), Hamster (92%), Panda (92%), Rabbit (92%), Opossum (92%), Bat (83%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2604. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human PTPRT. This antibody is expected to recognise both reported isoforms. Epitope corresponding to aa 58-69 of human PTPRT protein.
Immunohistochemistry: LS-B2604 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2604 was determined to be 5 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.