RPTP-Alpha antibody LS-A6795 is an unconjugated rabbit polyclonal antibody to human RPTP-Alpha (PTPRA). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Monkey, Rat (at least 90% immunogen sequence identity)
Also available conjugated with AP
Synthetic 17 amino acid peptide from extracellular domain of human PTPRA. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Rat (94%); Hamster, Dog, Bovine, Rabbit (88%); Mouse, Panda, Horse (82%).
Human PTPRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HLA-S (59%).
was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A8061
was determined to be 15 ug/ml.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.