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Anti-PTPN11 / SHP-2 / NS1 Antibody (N-Terminus) IHC-plus™ LS-B542

Note: This antibody replaces LS-C19630

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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PTPN11 / SHP-2 / NS1 Antibody LS-B542 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-PTPN11 antibody IHC of human skeletal muscle.
Immunohistochemistry
Anti-PTPN11 antibody IHC of human brain, cortex.
Immunofluorescence
Immunofluorescence of SHP2 in Human Skeletal Muscle cells with SHP2 antibody at 20 ug/ml.
Western blot
Western blot of SHP2 in mouse skeletal muscle tissue lysate with SHP2 antibody at (A) 0.5 and...

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Rabbit Polyclonal to Human PTPN11 / SHP-2 / NS1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human PTPN11 / SHP-2 / NS1
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot

Specificity and Use

PTPN11 / SHP-2 / NS1 antibody was raised against synthetic peptide from human PTPN11.
LS-E30266 - Liquid - 50 µg - $145.00
N-Terminus
14 amino acid peptide from near the amino terminus of human SHP2
Immunohistochemistry: LS-B542 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B542 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About PTPN11 / SHP-2 / NS1

Q06124 NM_002834 NP_002825.3

PTPN11 Antibody, Bptp-3 Antibody, BPTP3 Antibody, CFC Antibody, Csw Antibody, Corkscrew csw Antibody, Gene corkscrew Antibody, Phosphatase corkscrew Antibody, PTP-2C Antibody, SHP2 Antibody, PTP-1D Antibody, PTP2C Antibody, SHP-2 Antibody, SHPTP2 Antibody, Noonan syndrome 1 Antibody, SH-PTP2 Antibody, SH-PTP3 Antibody

SHP2, a NT2 non-receptor protein tyrosine phosphatases, contains two tandem SRC homology (SH2) domains at the N-terminus of the catalytic domain. Based on crystallographic studies, the SH2 domain is believed to function as a conformational switch which can bind and inhibit the phosphatase or bind phosphoprotein and activate the enzyme. SHP2 is thought to link growth factor receptors and other signalling proteins via protein-tyrosine phosphorylation.

Immunohistochemistry

Immunohistochemistry
Anti-PTPN11 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B542 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-PTPN11 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B542 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of SHP2 in Human Skeletal Muscle cells with SHP2 antibody at 20 ug/ml.

Western blot

Western blot
Western blot of SHP2 in mouse skeletal muscle tissue lysate with SHP2 antibody at (A) 0.5 and (B) 1 ug/ml.

Requested From: United States
Date Requested: 5/30/2015

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