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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PTP1B Antibody (Internal) LS‑C100983
PTP1B antibody LS-C100983 is an unconjugated rabbit polyclonal antibody to human PTP1B. Validated for IHC and WB.
Catalog
Size
Price
LS-C100983-50
50 µg
Unavailable

Popular PTP1B Products

Anti-PTPN1 / PTP1B antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7639 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry of paraffin-embedded mouse brain tissue.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemical analysis of PTP1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Monkey, Mouse, Rat, Dog, Pig, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Western blot of Phospho-PTP1B (S50) antibody
Species: Human, Monkey, Mouse, Rat
Applications: IHC, Western blot, ELISA
Western blot of PTP1B antibody
Species: Human, Monkey, Mouse, Rat
Applications: IHC, Western blot, ELISA

Product Description

PTP1B antibody LS-C100983 is an unconjugated rabbit polyclonal antibody to human PTP1B. Validated for IHC and WB.
About PTP1B
PTP1B is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. P18031 NM_002827 NP_002818.1

PTPN1 Antibody, PTP1B Antibody, PTP-1B Antibody, PTPase 1B Antibody


Specifications

Target
Human PTP1B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PTP1B antibody was raised against kLH conjugated synthetic peptide selected from the center region of human PTP1B.
Epitope
Internal
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PTP1B antibody (Center) LS-C100983, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PTP1B antibody (Center) LS-C100983, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot

Western blot of anti-PTP1B Ctr LS-C100983 in HepG2 cell line lysate (35 ug/lane). PTP1B Ctr(arrow) was detected using the purified Pab (1:40 dilution).
Western blot of anti-PTP1B Ctr LS-C100983 in HepG2 cell line lysate (35 ug/lane). PTP1B Ctr(arrow) was detected using the purified Pab (1:40 dilution).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PTP1B antibody (Center) LS-C100983, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PTP1B antibody (Center) LS-C100983, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot

Western blot of anti-PTP1B Ctr LS-C100983 in HepG2 cell line lysate (35 ug/lane). PTP1B Ctr(arrow) was detected using the purified Pab (1:40 dilution).
Western blot of anti-PTP1B Ctr LS-C100983 in HepG2 cell line lysate (35 ug/lane). PTP1B Ctr(arrow) was detected using the purified Pab (1:40 dilution).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PTP1B antibody (Center) LS-C100983, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PTP1B antibody (Center) LS-C100983, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot

Western blot of anti-PTP1B Ctr LS-C100983 in HepG2 cell line lysate (35 ug/lane). PTP1B Ctr(arrow) was detected using the purified Pab (1:40 dilution).
Western blot of anti-PTP1B Ctr LS-C100983 in HepG2 cell line lysate (35 ug/lane). PTP1B Ctr(arrow) was detected using the purified Pab (1:40 dilution).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PTP1B antibody (Center) LS-C100983, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PTP1B antibody (Center) LS-C100983, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot

Western blot of anti-PTP1B Ctr LS-C100983 in HepG2 cell line lysate (35 ug/lane). PTP1B Ctr(arrow) was detected using the purified Pab (1:40 dilution).
Western blot of anti-PTP1B Ctr LS-C100983 in HepG2 cell line lysate (35 ug/lane). PTP1B Ctr(arrow) was detected using the purified Pab (1:40 dilution).


Requested From: 
Date Requested: 6/24/2018

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