Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
PTK2B / PYK2 antibody was raised against synthetic peptide (KLH) containing. DI[pY]AE. in which pY corresponds to phosphotyrosine 402 of human Pyk2 (protein tyrosine kinase 2). The immunizing sequence is identical in human and rat.
Recognizes human phosphorylated Pyk2, MW: 116kD. Species cross-reactivity: Rat.
Suitable for use in ELISA and Immunoprecipitation/Western Blot. Not suitable for Western Blot (cross-reacts with phosphorylated FAK). Immunoprecipitation: 1 ug/ml detects phosphorylated Pyk2 in Pyk2 immunoprecipitate from RIPA lysates of PC12 cells treated with EGF, Bradykinin or KCl but not from untreated cell lysates.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation.