PYK2 antibody LS-C17523 is an unconjugated mouse monoclonal antibody to PYK2 (PTK2B) from human and rat. Validated for ELISA, IP and WB.
Human PTK2B / PYK2
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot
- Immunoprecipitation (1 µg/ml)
- (applications tested for the base form of this product only)
PTK2B / PYK2 antibody was raised against synthetic peptide (KLH) containing. DI[pY]AE. in which pY corresponds to phosphotyrosine 402 of human Pyk2 (protein tyrosine kinase 2). The immunizing sequence is identical in human and rat.
Recognizes human phosphorylated Pyk2, MW: 116kD. Species cross-reactivity: Rat.
Suitable for use in ELISA and Immunoprecipitation/Western Blot. Not suitable for Western Blot (cross-reacts with phosphorylated FAK). Immunoprecipitation: 1 ug/ml detects phosphorylated Pyk2 in Pyk2 immunoprecipitate from RIPA lysates of PC12 cells treated with EGF, Bradykinin or KCl but not from untreated cell lysates.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.