PYK2 antibody LS-C17521 is an unconjugated rabbit polyclonal antibody to PYK2 (PTK2B) from human and mouse. Validated for IP and WB.
Human PTK2B / PYK2
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
PTK2B / PYK2 antibody was raised against gST fusion protein containing aa726-863 of human Pyk2.
Recognizes Pyk2 at 116kD in mouse brain cytosol. Also detects a band at 40kD, which may be a degradation product. Does not cross-react with FAK. Species cross-reactivity: Human and mouse.
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit (HRP) secondary antibody and a chemiluminescence detection system. Pyk2 has a MW of approximately 116kD as calculated from the amino acid sequence deduced from the complete human cDNA. Immunoprecipitation: 4 ug detected Pyk2 from 500 ug of mouse brain cytosolic RIPA lysate.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.