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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PTK2B / PYK2 Antibody (aa726‑863) LS‑C17521
PYK2 antibody LS-C17521 is an unconjugated rabbit polyclonal antibody to PYK2 (PTK2B) from human and mouse. Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C17521-250
250 µg
$695

Popular PTK2B / PYK2 Products

Anti-PTK2B / PYK2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4093 dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-PTK2B / PYK2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1892 dilution 1:50.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PYK2 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-PTK2B / PYK2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7507 dilution 1:50.
Species: Rat, Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Western blot of lysates from mouse brain, mouse spleen, rat brain tissue (from left to right) with Ptk2b Antibody (P769). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
Species: Mouse, Rat
Applications: Western blot

Product Description

PYK2 antibody LS-C17521 is an unconjugated rabbit polyclonal antibody to PYK2 (PTK2B) from human and mouse. Validated for IP and WB.

Specifications

Target
Human PTK2B / PYK2
Synonyms
PTK2B, CADTK, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, Focal adhesion kinase 2, FRNK, FAK2, Proline-rich tyrosine kinase 2, Protein-tyrosine kinase 2-beta, Protein kinase B, Protein tyrosine kinase 2 beta, PYK2, RAFTK, CAK-beta, PKB, PTK
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
PTK2B / PYK2 antibody was raised against gST fusion protein containing aa726-863 of human Pyk2.
Epitope
aa726-863
Specificity
Recognizes Pyk2 at 116kD in mouse brain cytosol. Also detects a band at 40kD, which may be a degradation product. Does not cross-react with FAK. Species cross-reactivity: Human and mouse.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit (HRP) secondary antibody and a chemiluminescence detection system. Pyk2 has a MW of approximately 116kD as calculated from the amino acid sequence deduced from the complete human cDNA. Immunoprecipitation: 4 ug detected Pyk2 from 500 ug of mouse brain cytosolic RIPA lysate.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About PTK2B / PYK2
Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Q14289 NM_004103 NP_004094.3


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Requested From: United States
Date Requested: 10/22/2018

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