Rat PTGS2 / COX2 / COX-2
Rat, Human, Mouse
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot (2 - 4 µg/ml)
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Synthetic peptide corresponding to C-terminus of rat COX2. Cellular Localization: Cytoplasmic and membrane.
Recognizes rat COX2.
Immunohistochemistry: LS-B2145 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2145 was determined to be 5 ug/ml. Western Blot: 2-4 ug/ml for 2 hours at RT.
10 mM PBS, pH 7.6, 0.2% BSA, 15 mM sodium azide, before the addition of glycerol to 40%
Short term 4°C (no longer than 1 week); Long term -20°C; Aliquot to avoid freeze/thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.