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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PTGS2 / COX2 / COX‑2 Antibody (C‑Terminus) IHC‑plus™ LS‑B2145
Note: This antibody replaces LS-C22404
COX-2 antibody LS-B2145 is an unconjugated rabbit polyclonal antibody to COX-2 (PTGS2 / COX2) from human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2145-125
125 µl (0.1 mg/ml)
Unavailable

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Product Description

COX-2 antibody LS-B2145 is an unconjugated rabbit polyclonal antibody to COX-2 (PTGS2 / COX2) from human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About PTGS2 / COX2 / COX-2
Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. P35354 NM_000963 NP_000954.1

PTGS2 Antibody, COX-2 Antibody, Cyclooxygenase-2 Antibody, COX2 Antibody, Cyclooxygenase 2b Antibody, HCox-2 Antibody, GRIPGHS Antibody, PGH synthase 2 Antibody, PGHS-2 Antibody, PHS II Antibody, PHS-2 Antibody, Prostaglandin H2 synthase 2 Antibody, PGG/HS Antibody, Prostaglandin G/H synthase 2 Antibody


Specifications

Target
Rat PTGS2 / COX2 / COX-2
Host
Rabbit
Reactivity
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 - 4 µg/ml)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide corresponding to C-terminus of rat COX2. Cellular Localization: Cytoplasmic and membrane.
Epitope
C-Terminus
Specificity
Recognizes rat COX2.
Usage
Immunohistochemistry: LS-B2145 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2145 was determined to be 5 ug/ml. Western Blot: 2-4 ug/ml for 2 hours at RT.
Presentation
10 mM PBS, pH 7.6, 0.2% BSA, 15 mM sodium azide, before the addition of glycerol to 40%
Storage
Short term 4°C (no longer than 1 week); Long term -20°C; Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-COX-2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2145 concentration 5 ug/ml.
Anti-COX-2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2145 concentration 5 ug/ml.

Immunohistochemistry

Anti-COX-2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2145 concentration 5 ug/ml.
Anti-COX-2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2145 concentration 5 ug/ml.

Immunohistochemistry

Anti-COX-2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2145 concentration 5 ug/ml.
Anti-COX-2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2145 concentration 5 ug/ml.

Immunohistochemistry

Anti-COX-2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2145 concentration 5 ug/ml.
Anti-COX-2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2145 concentration 5 ug/ml.


Requested From: 
Date Requested: 6/23/2018

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