Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat, Human (tested or 100% immunogen sequence identity)
Western blot (1:1000 - 1:5000)
ELISA (1:10000 - 1:50000)
Specificity and Use
PTGER3 / EP3 antibody was raised against a 16aa synthetic peptide within the extracellular, N-terminal domain of Rat EP3 coupled to KLH.
Recognizes human EP3 Receptor. The human control peptide is located before the splicing point, therefore, it is conserved in all EP3 isoforms that differ at the C-terminus. Species sequence homology: rabbit and sheep-87%, bovine-86%, porcine-81%, rat and mouse EP3-68%. No significant homology of exist with either mouse or rat EP3 or other EP (1, 2 or 4) receptors.
Suitable for use in ELISA and Western Blot. ELISA: 1:10000-1:50000. Western Blot: 1:1000-1:5000.
40% glycerol, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
Receptor for prostaglandin E2 (PGE2); the EP3 receptor may be involved in inhibition of gastric acid secretion, modulation of neurotransmitter release in central and peripheral neurons, inhibition of sodium and water reabsorption in kidney tubulus and contraction in uterine smooth muscle. The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G-I proteins, and to an elevation of intracellular calcium.