Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Monkey, Bat, Horse, Rabbit (at least 90% immunogen sequence identity)
IHC - Paraffin (5 µg/ml)
ICC (1 - 2 µg/ml)
Western blot (1 - 2 µg/ml)
Specificity and Use
PTGER3 / EP3 antibody was raised against human EP3 receptor amino acids 308-327 (NQTSVEHCKTHTEKQKECNF). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Monkey, Bat, Panda, Horse (95%); Rabbit (90%); Elephant (85%); Bovine, Pig (80%).
The EP3 receptor appears to be expressed at low levels in many tissues and cell types, potentially making detection by immunochemical techniques difficult. Does not cross react with EP1, EP2 and EP4 receptors.
Receptor for prostaglandin E2 (PGE2); the EP3 receptor may be involved in inhibition of gastric acid secretion, modulation of neurotransmitter release in central and peripheral neurons, inhibition of sodium and water reabsorption in kidney tubulus and contraction in uterine smooth muscle. The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G-I proteins, and to an elevation of intracellular calcium.